TY - JOUR
T1 - WW6
T2 - An embryonic stem cell line with an inert genetic marker that can be traced in chimeras
AU - Ioffe, Ella
AU - Liu, Yun
AU - Bhaumik, Mantu
AU - Poirier, Francoise
AU - Factor, Stephen M.
AU - Stanley, Pamela
PY - 1995/8/1
Y1 - 1995/8/1
N2 - Mutant mice produced by gene targeting in embryonic stem (ES) cells often have a complex or embryonic lethal phenotype. In these cases, it would he helpful to identify tissues and cell types first affected in mutant embryos by following the contribution to chimeras of ES cells homozygous for the mutant allele. Although a number of strategies for following ES cell development in vivo have been reported, each has limitations that preclude its general application. In this paper, we describe ES cell lines that can be tracked to every nucleated cell type in chimeras at all developmental stages. These lines were derived from blastocysts of mice that carry an 11-Mb β- globin transgene on chromosome 3. The transgene is readily detected by DNA in situ hybridization, providing an inert, nuclear-localized marker whose presence is not affected by transcriptional or translational controls. The 'WW' series of ES lines possess the essential features of previously described ES lines, including giving rise to a preponderance of male chimeras, all of which have to date exhibited germ-line transmission. In addition, clones selected for single or double targeting events form strong chimeras, demonstrating the feasibility of using WW6 celts to identify phenotypes associated with the creation of a null mutant.
AB - Mutant mice produced by gene targeting in embryonic stem (ES) cells often have a complex or embryonic lethal phenotype. In these cases, it would he helpful to identify tissues and cell types first affected in mutant embryos by following the contribution to chimeras of ES cells homozygous for the mutant allele. Although a number of strategies for following ES cell development in vivo have been reported, each has limitations that preclude its general application. In this paper, we describe ES cell lines that can be tracked to every nucleated cell type in chimeras at all developmental stages. These lines were derived from blastocysts of mice that carry an 11-Mb β- globin transgene on chromosome 3. The transgene is readily detected by DNA in situ hybridization, providing an inert, nuclear-localized marker whose presence is not affected by transcriptional or translational controls. The 'WW' series of ES lines possess the essential features of previously described ES lines, including giving rise to a preponderance of male chimeras, all of which have to date exhibited germ-line transmission. In addition, clones selected for single or double targeting events form strong chimeras, demonstrating the feasibility of using WW6 celts to identify phenotypes associated with the creation of a null mutant.
KW - DNA in situ hybridization
UR - http://www.scopus.com/inward/record.url?scp=0029091809&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029091809&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.16.7357
DO - 10.1073/pnas.92.16.7357
M3 - Article
C2 - 7638196
AN - SCOPUS:0029091809
SN - 0027-8424
VL - 92
SP - 7357
EP - 7361
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 16
ER -