WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity

Andrew Xiao, Haitao Li, David Shechter, Sung Hee Ahn, Laura A. Fabrizio, Hediye Erdjument-Bromage, Satoko Ishibe-Murakami, Bin Wang, Paul Tempst, Kay Hofmann, Dinshaw J. Patel, Stephen J. Elledge, C. David Allis

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

DNA double-stranded breaks present a serious challenge for eukaryotic cells. The inability to repair breaks leads to genomic instability, carcinogenesis and cell death. During the double-strand break response, mammalian chromatin undergoes reorganization demarcated by H2A.X Ser 139 phosphorylation (γ-H2A.X). However, the regulation of γ-H2A.X phosphorylation and its precise role in chromatin remodelling during the repair process remain unclear. Here we report a new regulatory mechanism mediated by WSTF (Williams-Beuren syndrome transcription factor, also known as BAZ1B) - a component of the WICH complex (WSTF-ISWI ATP-dependent chromatin-remodelling complex). We show that WSTF has intrinsic tyrosine kinase activity by means of a domain that shares no sequence homology to any known kinase fold. We show that WSTF phosphorylates Tyr 142 of H2A.X, and that WSTF activity has an important role in regulating several events that are critical for the DNA damage response. Our work demonstrates a new mechanism that regulates the DNA damage response and expands our knowledge of domains that contain intrinsic tyrosine kinase activity.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalNature
Volume457
Issue number7225
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Chromatin Assembly and Disassembly
Protein-Tyrosine Kinases
DNA Damage
Phosphorylation
Williams Syndrome
Double-Stranded DNA Breaks
Genomic Instability
Eukaryotic Cells
Sequence Homology
Chromatin
Carcinogenesis
Cell Death
Transcription Factors
Phosphotransferases
Adenosine Triphosphate

ASJC Scopus subject areas

  • General

Cite this

Xiao, A., Li, H., Shechter, D., Ahn, S. H., Fabrizio, L. A., Erdjument-Bromage, H., ... Allis, C. D. (2009). WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. Nature, 457(7225), 57-62. https://doi.org/10.1038/nature07668

WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. / Xiao, Andrew; Li, Haitao; Shechter, David; Ahn, Sung Hee; Fabrizio, Laura A.; Erdjument-Bromage, Hediye; Ishibe-Murakami, Satoko; Wang, Bin; Tempst, Paul; Hofmann, Kay; Patel, Dinshaw J.; Elledge, Stephen J.; Allis, C. David.

In: Nature, Vol. 457, No. 7225, 01.01.2009, p. 57-62.

Research output: Contribution to journalArticle

Xiao, A, Li, H, Shechter, D, Ahn, SH, Fabrizio, LA, Erdjument-Bromage, H, Ishibe-Murakami, S, Wang, B, Tempst, P, Hofmann, K, Patel, DJ, Elledge, SJ & Allis, CD 2009, 'WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity', Nature, vol. 457, no. 7225, pp. 57-62. https://doi.org/10.1038/nature07668
Xiao A, Li H, Shechter D, Ahn SH, Fabrizio LA, Erdjument-Bromage H et al. WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. Nature. 2009 Jan 1;457(7225):57-62. https://doi.org/10.1038/nature07668
Xiao, Andrew ; Li, Haitao ; Shechter, David ; Ahn, Sung Hee ; Fabrizio, Laura A. ; Erdjument-Bromage, Hediye ; Ishibe-Murakami, Satoko ; Wang, Bin ; Tempst, Paul ; Hofmann, Kay ; Patel, Dinshaw J. ; Elledge, Stephen J. ; Allis, C. David. / WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. In: Nature. 2009 ; Vol. 457, No. 7225. pp. 57-62.
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