Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation

David M. Loeb, Ella Evron, Chirag B. Patel, Prem Mohini Sharma, Birunthi Niranjan, Lakjaya Buluwela, Sigmund A. Weitzman, Dorian Korz, Saraswati Sukumar

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.

Original languageEnglish (US)
Pages (from-to)921-925
Number of pages5
JournalCancer Research
Volume61
Issue number3
StatePublished - Feb 1 2001
Externally publishedYes

Fingerprint

Wilms' Tumor Genes
Wilms Tumor
Tumor Suppressor Genes
Carcinogens
Methylation
Breast Neoplasms
Neoplasms
Breast
Epithelium
Carcinoma
Tumor Suppressor Proteins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Loeb, D. M., Evron, E., Patel, C. B., Sharma, P. M., Niranjan, B., Buluwela, L., ... Sukumar, S. (2001). Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation. Cancer Research, 61(3), 921-925.

Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation. / Loeb, David M.; Evron, Ella; Patel, Chirag B.; Sharma, Prem Mohini; Niranjan, Birunthi; Buluwela, Lakjaya; Weitzman, Sigmund A.; Korz, Dorian; Sukumar, Saraswati.

In: Cancer Research, Vol. 61, No. 3, 01.02.2001, p. 921-925.

Research output: Contribution to journalArticle

Loeb, DM, Evron, E, Patel, CB, Sharma, PM, Niranjan, B, Buluwela, L, Weitzman, SA, Korz, D & Sukumar, S 2001, 'Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation', Cancer Research, vol. 61, no. 3, pp. 921-925.
Loeb, David M. ; Evron, Ella ; Patel, Chirag B. ; Sharma, Prem Mohini ; Niranjan, Birunthi ; Buluwela, Lakjaya ; Weitzman, Sigmund A. ; Korz, Dorian ; Sukumar, Saraswati. / Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation. In: Cancer Research. 2001 ; Vol. 61, No. 3. pp. 921-925.
@article{3d93fb4b8b2f459eb0085157b7efd36d,
title = "Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation",
abstract = "We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32{\%}) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.",
author = "Loeb, {David M.} and Ella Evron and Patel, {Chirag B.} and Sharma, {Prem Mohini} and Birunthi Niranjan and Lakjaya Buluwela and Weitzman, {Sigmund A.} and Dorian Korz and Saraswati Sukumar",
year = "2001",
month = "2",
day = "1",
language = "English (US)",
volume = "61",
pages = "921--925",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "3",

}

TY - JOUR

T1 - Wilms’ tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation

AU - Loeb, David M.

AU - Evron, Ella

AU - Patel, Chirag B.

AU - Sharma, Prem Mohini

AU - Niranjan, Birunthi

AU - Buluwela, Lakjaya

AU - Weitzman, Sigmund A.

AU - Korz, Dorian

AU - Sukumar, Saraswati

PY - 2001/2/1

Y1 - 2001/2/1

N2 - We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.

AB - We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=0035117177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035117177&partnerID=8YFLogxK

M3 - Article

C2 - 11221883

AN - SCOPUS:0035117177

VL - 61

SP - 921

EP - 925

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 3

ER -