Whole-genome sequencing and iPLEX MassARRAY genotyping map an ems-induced mutation affecting cell competition in drosophila melanogaster

Chang Hyun Lee, Gerard Rimesso, David M. Reynolds, Jinlu Cai, Nicholas E. Baker

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Cell competition, the conditional loss of viable genotypes only when surrounded by other cells, is a phenomenon observed in certain genetic mosaic conditions. We conducted a chemical mutagenesis and screen to recover new mutations that affect cell competition between wild-type and RpS3 heterozygous cells. Mutations were identified by whole-genome sequencing, making use of software tools that greatly facilitate the distinction between newly induced mutations and other sources of apparent sequence polymorphism, thereby reducing false-positive and false-negative identification rates. In addition, we utilized iPLEX MassARRAY for genotyping recombinant chromosomes. These approaches permitted the mapping of a new mutation affecting cell competition when only a single allele existed, with a phenotype assessed only in genetic mosaics, without the benefit of complementation with existing mutations, deletions, or duplications. These techniques expand the utility of chemical mutagenesis and whole-genome sequencing for mutant identification. We discuss mutations in the Atm and Xrpl genes identified in this screen.

Original languageEnglish (US)
Pages (from-to)3207-3217
Number of pages11
JournalG3: Genes, Genomes, Genetics
Volume6
Issue number10
DOIs
StatePublished - 2016

Keywords

  • Cell competition
  • Drosophila melanogaster
  • Flybook
  • Whole-genome sequencing
  • Xrp1
  • iPLEX MassARRAY

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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