White to beige conversion in PDE3B KO adipose tissue through activation of AMPK signaling and mitochondrial function

Youn Wook Chung, Faiyaz Ahmad, Yan Tang, Steven C. Hockman, Hyun Jung Kee, Karin Berger, Emilia Guirguis, Young Hun Choi, Dan M. Schimel, Angel M. Aponte, Sunhee Park, Eva Degerman, Vincent C. Manganiello

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Understanding mechanisms by which a population of beige adipocytes is increased in white adipose tissue (WAT) reflects a potential strategy in the fight against obesity and diabetes. Cyclic adenosine monophosphate (cAMP) is very important in the development of the beige phenotype and activation of its thermogenic program. To study effects of cyclic nucleotides on energy homeostatic mechanisms, mice were generated by targeted inactivation of cyclic nucleotide phosphodiesterase 3b (Pde3b) gene, which encodes PDE3B, an enzyme that catalyzes hydrolysis of cAMP and cGMP and is highly expressed in tissues that regulate energy homeostasis, including adipose tissue, liver, and pancreas. In epididymal white adipose tissue (eWAT) of PDE3B KO mice on a SvJ129 background, cAMP/protein kinase A (PKA) and AMP-activated protein kinase (AMPK) signaling pathways are activated, resulting in "browning" phenotype, with a smaller increases in body weight under high-fat diet, smaller fat deposits, increased β-oxidation of fatty acids (FAO) and oxygen consumption. Results reported here suggest that PDE3B and/or its downstream signaling partners might be important regulators of energy metabolism in adipose tissue, and potential therapeutic targets for treating obesity, diabetes and their associated metabolic disorders.

Original languageEnglish (US)
Article number40445
JournalScientific Reports
Volume7
DOIs
StatePublished - Jan 13 2017
Externally publishedYes

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AMP-Activated Protein Kinases
Cyclic AMP
Adipose Tissue
White Adipose Tissue
Cyclic Nucleotides
Obesity
Type 3 Cyclic Nucleotide Phosphodiesterases
Phenotype
High Fat Diet
Cyclic AMP-Dependent Protein Kinases
Oxygen Consumption
Energy Metabolism
Pancreas
Hydrolysis
Homeostasis
Fatty Acids
Fats
Body Weight
Liver
Enzymes

ASJC Scopus subject areas

  • General

Cite this

White to beige conversion in PDE3B KO adipose tissue through activation of AMPK signaling and mitochondrial function. / Chung, Youn Wook; Ahmad, Faiyaz; Tang, Yan; Hockman, Steven C.; Kee, Hyun Jung; Berger, Karin; Guirguis, Emilia; Choi, Young Hun; Schimel, Dan M.; Aponte, Angel M.; Park, Sunhee; Degerman, Eva; Manganiello, Vincent C.

In: Scientific Reports, Vol. 7, 40445, 13.01.2017.

Research output: Contribution to journalArticle

Chung, YW, Ahmad, F, Tang, Y, Hockman, SC, Kee, HJ, Berger, K, Guirguis, E, Choi, YH, Schimel, DM, Aponte, AM, Park, S, Degerman, E & Manganiello, VC 2017, 'White to beige conversion in PDE3B KO adipose tissue through activation of AMPK signaling and mitochondrial function', Scientific Reports, vol. 7, 40445. https://doi.org/10.1038/srep40445
Chung, Youn Wook ; Ahmad, Faiyaz ; Tang, Yan ; Hockman, Steven C. ; Kee, Hyun Jung ; Berger, Karin ; Guirguis, Emilia ; Choi, Young Hun ; Schimel, Dan M. ; Aponte, Angel M. ; Park, Sunhee ; Degerman, Eva ; Manganiello, Vincent C. / White to beige conversion in PDE3B KO adipose tissue through activation of AMPK signaling and mitochondrial function. In: Scientific Reports. 2017 ; Vol. 7.
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