What Have We Learned from Glycosyltransferase Knockouts in Mice?

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations

Abstract

There are five major classes of glycan including N- and O-glycans, glycosaminoglycans, glycosphingolipids, and glycophosphatidylinositol anchors, all expressed at the molecular frontier of each mammalian cell. Numerous biological consequences of altering the expression of mammalian glycans are understood at a mechanistic level, but many more remain to be characterized. Mouse mutants with deleted, defective, or misexpressed genes that encode activities necessary for glycosylation have led the way to identifying key functions of glycans in biology. However, with the advent of exome sequencing, humans with mutations in genes involved in glycosylation are also revealing specific requirements for glycans in mammalian development. The aim of this review is to summarize glycosylation genes that are necessary for mouse embryonic development, pathway-specific glycosylation genes whose deletion leads to postnatal morbidity, and glycosylation genes for which effects are mild, but perturbation of the organism may reveal functional consequences. General strategies for generating and interpreting the phenotype of mice with glycosylation defects are discussed in relation to human congenital disorders of glycosylation (CDG).

Original languageEnglish (US)
Pages (from-to)3166-3182
Number of pages17
JournalJournal of Molecular Biology
Volume428
Issue number16
DOIs
StatePublished - Aug 14 2016

Keywords

  • glycosidase
  • glycosylation
  • glycosyltransferase
  • mouse mutants
  • nucleotide sugar

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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