What can structure tell us about in vivo function? The case of aminoglycoside-resistance genes

M. Vetting, S. L. Roderick, Subray Hegde, S. Magnet, John S. Blanchard

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Resistance to antibiotics used in the treatment of bacterial infections is an expanding clinical problem. Aminoglycosides, one of the oldest classes of natural product antibiotics, exert their bactericidal effect as the result of inhibiting bacterial protein synthesis by binding to the acceptor site of the 30 S ribosomal subunit. The most common mechanism of clinical resistance to aminoglycosides results from the expression of enzymes that covalently modify the aminoglycoside. We will discuss the enzymology and structure of two representative chromosomally encoded aminoglycoside N-acetyltransferases, Mycabacterium tuberculasis AAC(2′)-lc and Salmanella enterica AAC(6′)-ly, and speculate about their possible physiological function and substrates.

Original languageEnglish (US)
Pages (from-to)520-522
Number of pages3
JournalBiochemical Society Transactions
Volume31
Issue number3
DOIs
StatePublished - Jun 2003

Fingerprint

Aminoglycosides
aminoglycoside 2'-N-acetyltransferase
Genes
Ribosome Subunits
Bacterial Proteins
Microbial Drug Resistance
Anti-Bacterial Agents
Biological Products
Bacterial Infections
Protein Binding
Acetyltransferases
Enzymes
Substrates

Keywords

  • Acetyltransferase
  • Aminoglycoside
  • Antibiotic resistance
  • Mycobacterium tuberculosis
  • Salmonella enterica

ASJC Scopus subject areas

  • Biochemistry

Cite this

What can structure tell us about in vivo function? The case of aminoglycoside-resistance genes. / Vetting, M.; Roderick, S. L.; Hegde, Subray; Magnet, S.; Blanchard, John S.

In: Biochemical Society Transactions, Vol. 31, No. 3, 06.2003, p. 520-522.

Research output: Contribution to journalArticle

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AU - Blanchard, John S.

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