TY - JOUR
T1 - Wharton’s Jelly Transplantation Improves Neurologic Function in a Rat Model of Traumatic Brain Injury
AU - Cheng, Tian
AU - Yang, Bo
AU - Li, Dongpeng
AU - Ma, Shanshan
AU - Tian, Yi
AU - Qu, Ruina
AU - Zhang, Wenjin
AU - Zhang, Yanting
AU - Hu, Kai
AU - Guan, Fangxia
AU - Wang, Jian
N1 - Publisher Copyright:
© 2015, Springer Science+Business Media New York.
PY - 2015/7/25
Y1 - 2015/7/25
N2 - Traumatic brain injury (TBI), which can lead to disability, dysfunction, and even death, is a prominent health problem worldwide. Effective therapy for this serious and debilitating condition is needed. Human umbilical cord matrix, known as Wharton’s jelly (WJ), provides a natural, interface scaffold that is enriched in mesenchymal stem cells. In this study, we tested the efficacy of WJ tissue transplantation in a weight-drop model of TBI in rats. WJ tissue was cultured and transplanted into the injury site 24 h after TBI. The modified neurologic severity score, body weight, brain edema, and lesion volume were evaluated at various time points after TBI. Cognitive behavior was assessed by the novel object recognition test and the Morris water maze test. Expression of brain-derived neurotrophic factor (BDNF) in the perilesional brain area was measured at day 14 after TBI. We found that WJ tissue transplantation lessened TBI-induced brain edema (day 3), reduced lesion volume (day 28), improved neurologic function (days 21–28), and promoted memory and cognitive recovery. Additionally, expression of BDNF mRNA and protein was higher in WJ tissue-treated rats than in sham-operated or vehicle-treated rats. These data suggest that WJ tissue transplantation can reduce TBI-induced brain injury and may have therapeutic potential for the treatment of TBI.
AB - Traumatic brain injury (TBI), which can lead to disability, dysfunction, and even death, is a prominent health problem worldwide. Effective therapy for this serious and debilitating condition is needed. Human umbilical cord matrix, known as Wharton’s jelly (WJ), provides a natural, interface scaffold that is enriched in mesenchymal stem cells. In this study, we tested the efficacy of WJ tissue transplantation in a weight-drop model of TBI in rats. WJ tissue was cultured and transplanted into the injury site 24 h after TBI. The modified neurologic severity score, body weight, brain edema, and lesion volume were evaluated at various time points after TBI. Cognitive behavior was assessed by the novel object recognition test and the Morris water maze test. Expression of brain-derived neurotrophic factor (BDNF) in the perilesional brain area was measured at day 14 after TBI. We found that WJ tissue transplantation lessened TBI-induced brain edema (day 3), reduced lesion volume (day 28), improved neurologic function (days 21–28), and promoted memory and cognitive recovery. Additionally, expression of BDNF mRNA and protein was higher in WJ tissue-treated rats than in sham-operated or vehicle-treated rats. These data suggest that WJ tissue transplantation can reduce TBI-induced brain injury and may have therapeutic potential for the treatment of TBI.
KW - BDNF
KW - Cognition
KW - Transplantation
KW - Traumatic brain injury
KW - Wharton’s jelly
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UR - http://www.scopus.com/inward/citedby.url?scp=84931957079&partnerID=8YFLogxK
U2 - 10.1007/s10571-015-0159-9
DO - 10.1007/s10571-015-0159-9
M3 - Article
C2 - 25638565
AN - SCOPUS:84931957079
SN - 0272-4340
VL - 35
SP - 641
EP - 649
JO - Cellular and Molecular Neurobiology
JF - Cellular and Molecular Neurobiology
IS - 5
ER -
