Western analysis of intracellular interleukin-8 in human mononuclear leukocytes

Veronika Miskolci; Louis Hodgson; Dianne Cox; Ivana Vancurova

doi:10.1007/978-1-4939-0928-5_26

Western analysis of intracellular interleukin-8 in human mononuclear leukocytes

Veronika Miskolci, Louis Hodgson, Dianne Cox, Ivana Vancurova

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Most cytokines are stored in the cytoplasm until their release into the extracellular environment; however, some cytokines have been reported to localize in the nucleus. Traditional whole cell extract preparation does not provide information about the intracellular localization of cytokines. Here, we describe how to prepare cytoplasmic and nuclear extracts that can be analyzed by immunoblotting. While in this chapter we use this method to analyze intracellular localization of interleukin-8 (IL-8) in human mononuclear leukocytes, this protocol is adaptable to any cell type or protein of interest.

Original language	English (US)
Pages (from-to)	285-293
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1172
DOIs	https://doi.org/10.1007/978-1-4939-0928-5_26
State	Published - 2014
Externally published	Yes

Keywords

Cytokines
Cytoplasmic extract
Immunoblotting
Interleukin-8
Intracellular localization
Nuclear extract

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-0928-5_26

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title = "Western analysis of intracellular interleukin-8 in human mononuclear leukocytes",

abstract = "Most cytokines are stored in the cytoplasm until their release into the extracellular environment; however, some cytokines have been reported to localize in the nucleus. Traditional whole cell extract preparation does not provide information about the intracellular localization of cytokines. Here, we describe how to prepare cytoplasmic and nuclear extracts that can be analyzed by immunoblotting. While in this chapter we use this method to analyze intracellular localization of interleukin-8 (IL-8) in human mononuclear leukocytes, this protocol is adaptable to any cell type or protein of interest.",

keywords = "Cytokines, Cytoplasmic extract, Immunoblotting, Interleukin-8, Intracellular localization, Nuclear extract",

author = "Veronika Miskolci and Louis Hodgson and Dianne Cox and Ivana Vancurova",

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doi = "10.1007/978-1-4939-0928-5_26",

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T1 - Western analysis of intracellular interleukin-8 in human mononuclear leukocytes

AU - Miskolci, Veronika

AU - Hodgson, Louis

AU - Cox, Dianne

AU - Vancurova, Ivana

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Most cytokines are stored in the cytoplasm until their release into the extracellular environment; however, some cytokines have been reported to localize in the nucleus. Traditional whole cell extract preparation does not provide information about the intracellular localization of cytokines. Here, we describe how to prepare cytoplasmic and nuclear extracts that can be analyzed by immunoblotting. While in this chapter we use this method to analyze intracellular localization of interleukin-8 (IL-8) in human mononuclear leukocytes, this protocol is adaptable to any cell type or protein of interest.

AB - Most cytokines are stored in the cytoplasm until their release into the extracellular environment; however, some cytokines have been reported to localize in the nucleus. Traditional whole cell extract preparation does not provide information about the intracellular localization of cytokines. Here, we describe how to prepare cytoplasmic and nuclear extracts that can be analyzed by immunoblotting. While in this chapter we use this method to analyze intracellular localization of interleukin-8 (IL-8) in human mononuclear leukocytes, this protocol is adaptable to any cell type or protein of interest.

KW - Cytokines

KW - Cytoplasmic extract

KW - Immunoblotting

KW - Interleukin-8

KW - Intracellular localization

KW - Nuclear extract

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EP - 293

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Western analysis of intracellular interleukin-8 in human mononuclear leukocytes

Abstract

Keywords

ASJC Scopus subject areas

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