Western analysis of intracellular interleukin-8 in human mononuclear leukocytes

Veronika Miskolci, Louis Hodgson, Dianne Cox, Ivana Vancurova

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Most cytokines are stored in the cytoplasm until their release into the extracellular environment; however, some cytokines have been reported to localize in the nucleus. Traditional whole cell extract preparation does not provide information about the intracellular localization of cytokines. Here, we describe how to prepare cytoplasmic and nuclear extracts that can be analyzed by immunoblotting. While in this chapter we use this method to analyze intracellular localization of interleukin-8 (IL-8) in human mononuclear leukocytes, this protocol is adaptable to any cell type or protein of interest.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume1172
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Mononuclear Leukocytes
Interleukin-8
Cytokines
Cell Extracts
Immunoblotting
Cytoplasm
Proteins

Keywords

  • Cytokines
  • Cytoplasmic extract
  • Immunoblotting
  • Interleukin-8
  • Intracellular localization
  • Nuclear extract

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Western analysis of intracellular interleukin-8 in human mononuclear leukocytes. / Miskolci, Veronika; Hodgson, Louis; Cox, Dianne; Vancurova, Ivana.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1172, 2014, p. 285-293.

Research output: Contribution to journalArticle

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