TY - JOUR
T1 - Water channel-carrying vesicles in the rat IMCD contain cellubrevin
AU - Franki, N.
AU - Macaluso, F.
AU - Schubert, W.
AU - Gunther, L.
AU - Hays, R. M.
PY - 1995
Y1 - 1995
N2 - Antidiuretic hormone (arginine vasopressin) induces a cyclic process of docking, fusion, and endocytosis of water channel-containing vesicles in the collecting duct. There is now evidence that docking and endocytosis are mediated by an array of proteins associated with vesicles and target membranes. In recent studies, we have shown that cellubrevin, a member of the vesicle-associated membrane protein family, as well as other docking proteins, are expressed in the rat inner medullary collecting duct. We now show by immunogold electron microscopy that cellubrevin is present on vesicles containing water channels, that it is associated with both coated and uncoated vesicles, and that it is present on the apical membrane. Cellubrevin, therefore, is in a position to mediate one or more steps in arginine vasopressin-induced water channel cycling.
AB - Antidiuretic hormone (arginine vasopressin) induces a cyclic process of docking, fusion, and endocytosis of water channel-containing vesicles in the collecting duct. There is now evidence that docking and endocytosis are mediated by an array of proteins associated with vesicles and target membranes. In recent studies, we have shown that cellubrevin, a member of the vesicle-associated membrane protein family, as well as other docking proteins, are expressed in the rat inner medullary collecting duct. We now show by immunogold electron microscopy that cellubrevin is present on vesicles containing water channels, that it is associated with both coated and uncoated vesicles, and that it is present on the apical membrane. Cellubrevin, therefore, is in a position to mediate one or more steps in arginine vasopressin-induced water channel cycling.
KW - antidiuretic hormone
KW - aquaporins
KW - arginine vasopressin
KW - inner medullary collecting duct
KW - vesicle-associated membrane proteins
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U2 - 10.1152/ajpcell.1995.269.3.c797
DO - 10.1152/ajpcell.1995.269.3.c797
M3 - Article
C2 - 7573412
AN - SCOPUS:0028830898
SN - 0363-6143
VL - 269
SP - C797-C801
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 3 38-3
ER -