TY - JOUR
T1 - VH3 gene expression in children with HIV infection
AU - Chang, Qing
AU - Abadi, Jacobo
AU - Rosenberg, Michael
AU - Pirofski, Liise Anne
N1 - Funding Information:
This work was supported by NIH AI35370, AI45459 and AI44374 (LP) and NIH T32AI07501 (QC).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/11
Y1 - 2004/11
N2 - Objective. To study immunoglobulin heavy chain variable region (VH) gene expression in HIV-uninfected (HIV-) and HIV-infected (HIV+) children. Methods. A retrospective, observational study was performed by PCR-ELISA to examine IgM and IgG VH gene family expression among peripheral CD19-positive B cells. The subjects were 10 HIV+children with, and 11 HIV+children without a history of invasive pneumococcal disease (IPD) and 12 HIV- children. The ages of the HIV+and HIV- children, and the CD4 lymphocyte counts and viral loads (VL) of the HIV+/IPD- and HIV+/IPD- children were statistically similar. Rearranged VH3 gene libraries were constructed from 3 HIV+/IPD- children to examine individual gene expression. Results. The proportion of IgG VH3 expression among HIV+/IPD- was lower than that of HIV- children, but the proportion of IgG VH3 expression among HIV+/IPD+ children was not significantly different from that of HIV- children. IgG VH3 expression was positively, whereas IgM VH3 expression was inversely correlated with the VL among HIV+/IPD+, but not HIV+/IPD- children. IgM VH gene family expression did not differ between HIV+and HIV- children. Conclusion. HIV+ children manifest qualitative and quantitative differences in VH3 expression, which may be influenced by IPD.
AB - Objective. To study immunoglobulin heavy chain variable region (VH) gene expression in HIV-uninfected (HIV-) and HIV-infected (HIV+) children. Methods. A retrospective, observational study was performed by PCR-ELISA to examine IgM and IgG VH gene family expression among peripheral CD19-positive B cells. The subjects were 10 HIV+children with, and 11 HIV+children without a history of invasive pneumococcal disease (IPD) and 12 HIV- children. The ages of the HIV+and HIV- children, and the CD4 lymphocyte counts and viral loads (VL) of the HIV+/IPD- and HIV+/IPD- children were statistically similar. Rearranged VH3 gene libraries were constructed from 3 HIV+/IPD- children to examine individual gene expression. Results. The proportion of IgG VH3 expression among HIV+/IPD- was lower than that of HIV- children, but the proportion of IgG VH3 expression among HIV+/IPD+ children was not significantly different from that of HIV- children. IgG VH3 expression was positively, whereas IgM VH3 expression was inversely correlated with the VL among HIV+/IPD+, but not HIV+/IPD- children. IgM VH gene family expression did not differ between HIV+and HIV- children. Conclusion. HIV+ children manifest qualitative and quantitative differences in VH3 expression, which may be influenced by IPD.
KW - Antibody repertoire
KW - HIV infection
KW - Highly active anti-retroviral therapy
KW - Invasive pneumococcal disease
KW - Pneumococcal vaccines
KW - V3 gene family
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U2 - 10.1016/j.jinf.2004.04.007
DO - 10.1016/j.jinf.2004.04.007
M3 - Article
C2 - 15474624
AN - SCOPUS:5044242651
SN - 0163-4453
VL - 49
SP - 274
EP - 282
JO - Journal of Infection
JF - Journal of Infection
IS - 4
ER -