VH3 gene expression in children with HIV infection

Qing Chang, Jacobo Abadi, Michael Rosenberg, Liise Anne Pirofski

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objective. To study immunoglobulin heavy chain variable region (VH) gene expression in HIV-uninfected (HIV-) and HIV-infected (HIV+) children. Methods. A retrospective, observational study was performed by PCR-ELISA to examine IgM and IgG VH gene family expression among peripheral CD19-positive B cells. The subjects were 10 HIV+children with, and 11 HIV+children without a history of invasive pneumococcal disease (IPD) and 12 HIV- children. The ages of the HIV+and HIV- children, and the CD4 lymphocyte counts and viral loads (VL) of the HIV+/IPD- and HIV+/IPD- children were statistically similar. Rearranged VH3 gene libraries were constructed from 3 HIV+/IPD- children to examine individual gene expression. Results. The proportion of IgG VH3 expression among HIV+/IPD- was lower than that of HIV- children, but the proportion of IgG VH3 expression among HIV+/IPD+ children was not significantly different from that of HIV- children. IgG VH3 expression was positively, whereas IgM VH3 expression was inversely correlated with the VL among HIV+/IPD+, but not HIV+/IPD- children. IgM VH gene family expression did not differ between HIV+and HIV- children. Conclusion. HIV+ children manifest qualitative and quantitative differences in VH3 expression, which may be influenced by IPD.

Original languageEnglish (US)
Pages (from-to)274-282
Number of pages9
JournalJournal of Infection
Volume49
Issue number4
DOIs
Publication statusPublished - Nov 1 2004

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Keywords

  • Antibody repertoire
  • HIV infection
  • Highly active anti-retroviral therapy
  • Invasive pneumococcal disease
  • Pneumococcal vaccines
  • V3 gene family

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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