Voriconazole inhibits fungal growth without impairing antigen presentation or T-cell activation

Heather L. Van Epps, Marta Feldmesser, Eric G. Pamer

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Invasive aspergillosis (IA) is the most common life-threatening invasive mold infection worldwide. The principal therapy for IA is amphotericin B, despite its known toxicity and immunosuppressive side effects. Studies in animal models of IA suggest a role for T lymphocytes in the pathology of the disease, although the precise role for Aspergillus-specific T cells remains undefined. The isolation and characterization of T lymphocytes in animal models of IA are hampered by the rapid outgrowth of the fungus in cultures derived from infected organs. In the present study, we tested the abilities of the antifungal drugs caspofungin acetate and voriconazole to inhibit fungal growth in vitro as a means of maintaining cultures of T cells from Aspergillus-infected mice. We demonstrate that while both antifungal drugs are inhibitory, only voriconazole completely inhibited fungal growth, allowing long-term maintenance of T-cell cultures. In addition, voriconazole had no inhibitory effect on the activation and maturation of dendritic cells or the proliferation of T lymphocytes. Thus, voriconazole appears to be a promising agent for use in in vitro studies of Aspergillus-specific T lymphocytes in animal models of IA.

Original languageEnglish (US)
Pages (from-to)1818-1823
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume47
Issue number6
DOIs
StatePublished - Jun 1 2003

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Voriconazole inhibits fungal growth without impairing antigen presentation or T-cell activation'. Together they form a unique fingerprint.

  • Cite this