Volume measurements in cultured primary astrocytes

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Damage to the central nervous system (CNS) is selective, likely reflecting the intrinsic properties of Â-individual cell types. Targets of chemical injury are diverse hence assessing neurotoxicity is extremely difficult. Overcoming this obstacle requires a general screen or "marker" for injury that reflects cellular damage. The "marker" must be reliable and represent a biochemical event which broadly reflects cellular stress and damage. One such "marker" is cell swelling; it occurs in response to a diversity of insults, such as physical damage, disease (ischemia, trauma, and hypoxia), and chemicals (methylmercury, lead, 1,3-dinitrobenzene, and triethyltin). In astrocytes, a type of glia, astrocytic swelling can be measured with several methods. Commonly, freshly isolated astrocytes are grown to confluence on coverslips, a period requiring 3 weeks in culture. At this time, astrocytic volume can be measured using either an impedance technique or 3-O-methyl-d-glucose to assess cell volume. This review will briefly detail these methods and provide insight into molecular mechanisms associated with cell swelling and the ensuing regulatory decrease (RVD).

Original languageEnglish (US)
Title of host publicationIn Vitro Neurotoxicology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages391-402
Number of pages12
ISBN (Print)9781617791697
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume758
ISSN (Print)1064-3745

Keywords

  • 3-O-methyl-d-glucose
  • Astrocytes
  • Electrical resistance
  • Neurotoxicity
  • Swelling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Aschner, M. (2011). Volume measurements in cultured primary astrocytes. In In Vitro Neurotoxicology: Methods and Protocols (pp. 391-402). (Methods in Molecular Biology; Vol. 758). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-170-3_26