TY - JOUR
T1 - Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver
AU - Moreno, A. P.
AU - de Carvalho, A. C.
AU - Verselis, V.
AU - Eghbali, B.
AU - Spray, D. C.
N1 - Funding Information:
We thank Dr. M. V. L. Bennett for comments on an earlier draft of this manuscript. This work was supported in part by National Institutes of Health grants HL 34479 and NS 16524 to David C. Spray, NS 07512 (P.I.: M. V. L. Bennett, subproject to David C. Spray), and a National Science Foundation Binational grant to Antonio C. Campos de Carvalho and David C. Spray.
PY - 1991
Y1 - 1991
N2 - We report here experiments undertaken in pairs of hepatocytes that demonstrate a marked voltage sensivity of junctional conductance and, thus, contradict earlier findings reported by this laboratory (Spray, D.C., R.D.ginzberg, E.A., E. A. Morales, Z. Gatmaitan and I.M. Arias, 1986, J. Cell Biol. 101:135–144; Spray C.D. R.L. White, A.C. Campos de Carvalho, and M.V.L. Bennett. 1984. Biophys. J. 45:219–230) and by others (Dahl, G., T. Moller, D. Paul, R. Voellmy, and R. Werner. 1987. Science [Wash. DC] 236:1290–1293; Riverdin, E.C., and R. Weingart. 1988. Am. J. Physiol. 254:C226-C234). Expression in exogenous systems, lipid bilayers in which fragments of isolated gap junction membranes were incorporated (Young, J.D.-E., Z. Cohn, and N.B. Gilula. 1987. Cell. 48:733–743.) and noncommunicating cells transfected with connexin32 cDNA (Eghbali, B., J.A. Kessler, and D.C. Spray. 1990. Proc. Natl. Acad. Sci. USA. 87:1328–1331), support these findings and indicate that the voltage-dependent channel is composed of connexin32, the major gap junction protein of rat liver (Paul, D. 1986. J. Cell Biol. 103:123–134).
AB - We report here experiments undertaken in pairs of hepatocytes that demonstrate a marked voltage sensivity of junctional conductance and, thus, contradict earlier findings reported by this laboratory (Spray, D.C., R.D.ginzberg, E.A., E. A. Morales, Z. Gatmaitan and I.M. Arias, 1986, J. Cell Biol. 101:135–144; Spray C.D. R.L. White, A.C. Campos de Carvalho, and M.V.L. Bennett. 1984. Biophys. J. 45:219–230) and by others (Dahl, G., T. Moller, D. Paul, R. Voellmy, and R. Werner. 1987. Science [Wash. DC] 236:1290–1293; Riverdin, E.C., and R. Weingart. 1988. Am. J. Physiol. 254:C226-C234). Expression in exogenous systems, lipid bilayers in which fragments of isolated gap junction membranes were incorporated (Young, J.D.-E., Z. Cohn, and N.B. Gilula. 1987. Cell. 48:733–743.) and noncommunicating cells transfected with connexin32 cDNA (Eghbali, B., J.A. Kessler, and D.C. Spray. 1990. Proc. Natl. Acad. Sci. USA. 87:1328–1331), support these findings and indicate that the voltage-dependent channel is composed of connexin32, the major gap junction protein of rat liver (Paul, D. 1986. J. Cell Biol. 103:123–134).
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U2 - 10.1016/S0006-3495(91)82305-0
DO - 10.1016/S0006-3495(91)82305-0
M3 - Article
C2 - 1648416
AN - SCOPUS:0025761630
VL - 59
SP - 920
EP - 925
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 4
ER -