Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver

A. P. Moreno, A. C. Campos de Carvalho, Vytautas Verselis, B. Eghbali, David C. Spray

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Abstract

We report here experiments undertaken in pairs of hepatocytes that demonstrate a marked voltage sensitivity of junctional conductance and, thus, contradict earlier findings reported by this laboratory (Spray, D. C., R. D. Ginzberg, E. A. Morales, Z. Gatmaitan, and I. M. Arias. 1986. J. Cell Biol. 101:135-144; Spray, D. C., R. L. White, A. C. Campos de Carvalho, and M. V. L. Bennett. 1984. Biophys. J. 45:219-230) and by others (Dahl, G., T. Moller, D. Paul, R. Voellmy, and R. Werner. 1987. Science [Wash. DC.] 236:1290-1293; Riverdin, E. C., and R. Weingart. 1988. Am. J. Physiol. 254:C226-C234). Expression in exogenous systems, lipid bilayers in which fragments of isolated gap junction membranes were incorporated (Young, J. D.-E., Z. Cohn, and N. B. Gilula. 1987. Cell. 48:733-743.) and noncommunicating cells transfected with connexin32 cDNA (Eghbali, B., J. A. Kessler, and D. C. Spray. 1990. Proc. Natl. Acad. Sci. USA. 87:1328-1331), support these findings and indicate that the voltage-dependent channel is composed of connexin32, the major gap junction protein of rat liver (Paul, D. 1986. J. Cell Biol. 103:123-134).

Original languageEnglish (US)
Pages (from-to)920-925
Number of pages6
JournalBiophysical Journal
Volume59
Issue number4
StatePublished - 1991

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Connexins
Gap Junctions
Liver
Lipid Bilayers
Hepatocytes
Complementary DNA
Membranes

ASJC Scopus subject areas

  • Biophysics

Cite this

Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver. / Moreno, A. P.; Campos de Carvalho, A. C.; Verselis, Vytautas; Eghbali, B.; Spray, David C.

In: Biophysical Journal, Vol. 59, No. 4, 1991, p. 920-925.

Research output: Contribution to journal › Article

Moreno, AP, Campos de Carvalho, AC, Verselis, V, Eghbali, B & Spray, DC 1991, 'Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver', Biophysical Journal, vol. 59, no. 4, pp. 920-925.
Moreno AP, Campos de Carvalho AC, Verselis V, Eghbali B, Spray DC. Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver. Biophysical Journal. 1991;59(4):920-925.
Moreno, A. P. ; Campos de Carvalho, A. C. ; Verselis, Vytautas ; Eghbali, B. ; Spray, David C. / Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver. In: Biophysical Journal. 1991 ; Vol. 59, No. 4. pp. 920-925.
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