Vitamin D regulates steroidogenesis and insulin-like growth factor binding protein-1 (IGFBP-1) production in human ovarian cells

G. Parikh, M. Varadinova, P. Suwandhi, T. Araki, Z. Rosenwaks, L. Poretsky, D. Seto-Young

Research output: Contribution to journalArticle

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Abstract

Vitamin D Receptor (VDR) is expressed in both animal and human ovarian tissue, however, the role of vitamin D in human ovarian steroidogenesis is unknown. Cultured human ovarian cells were incubated in tissue culture medium supplemented with appropriate substrates, with or without 50pM150pM or 50nM150nM of 1,25-(OH)2D3, and in the presence or absence of insulin. Progesterone, testosterone, estrone, estradiol, and IGFBP-1 concentrations in conditioned tissue culture medium were measured. Vitamin D receptor was present in human ovarian cells. 1,25-(OH)2D3 stimulated progesterone production by 13% (p<0.001), estradiol production by 9% (p<0.02), and estrone production by 21% (p<0.002). Insulin and 1,25-(OH)2D3 acted synergistically to increase estradiol production by 60% (p<0.005). 1,25-(OH)2D3 alone stimulated IGFBP-1 production by 24% (p<0.001), however, in the presence of insulin, 1,25-(OH)2D3 enhanced insulin-induced inhibition of IGFBP-1 production by 13% (p<0.009). Vitamin D stimulates ovarian steroidogenesis and IGFBP-1 production in human ovarian cells likely acting via vitamin D receptor. Insulin and vitamin D synergistically stimulate estradiol production. Vitamin D also enhances inhibitory effect of insulin on IGFBP-1 production.

Original languageEnglish (US)
Pages (from-to)754-757
Number of pages4
JournalHormone and Metabolic Research
Volume42
Issue number10
DOIs
StatePublished - 2010

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Insulin-Like Growth Factor Binding Protein 1
Vitamin D
Insulin
Calcitriol Receptors
Estradiol
Estrone
Progesterone
Tissue culture
Culture Media
Conditioned Culture Medium
Testosterone
Animals
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Vitamin D regulates steroidogenesis and insulin-like growth factor binding protein-1 (IGFBP-1) production in human ovarian cells. / Parikh, G.; Varadinova, M.; Suwandhi, P.; Araki, T.; Rosenwaks, Z.; Poretsky, L.; Seto-Young, D.

In: Hormone and Metabolic Research, Vol. 42, No. 10, 2010, p. 754-757.

Research output: Contribution to journalArticle

Parikh, G, Varadinova, M, Suwandhi, P, Araki, T, Rosenwaks, Z, Poretsky, L & Seto-Young, D 2010, 'Vitamin D regulates steroidogenesis and insulin-like growth factor binding protein-1 (IGFBP-1) production in human ovarian cells', Hormone and Metabolic Research, vol. 42, no. 10, pp. 754-757. https://doi.org/10.1055/s-0030-1262837
Parikh, G. ; Varadinova, M. ; Suwandhi, P. ; Araki, T. ; Rosenwaks, Z. ; Poretsky, L. ; Seto-Young, D. / Vitamin D regulates steroidogenesis and insulin-like growth factor binding protein-1 (IGFBP-1) production in human ovarian cells. In: Hormone and Metabolic Research. 2010 ; Vol. 42, No. 10. pp. 754-757.
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