TY - JOUR
T1 - Visualization of podocyte substructure with structured illumination microscopy (SIM)
T2 - A new approach to nephrotic disease
AU - Pullman, James M.
AU - Nylk, Jonathan
AU - Campbell, Elaine C.
AU - Gunn-Moore, Frank J.
AU - Prystowsky, Michael B.
AU - Dholakia, Kishan
N1 - Publisher Copyright:
© 2016 Optical Society of America.
PY - 2016/1/6
Y1 - 2016/1/6
N2 - A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease.
AB - A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease.
KW - Fluorescence microscopy
KW - Medical and biological imaging
KW - Medical optics and biotechnology
KW - Microscopy
KW - Optical diagnostics for medicine
KW - Optical pathology
KW - Superresolution
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U2 - 10.1364/BOE.7.000302
DO - 10.1364/BOE.7.000302
M3 - Article
AN - SCOPUS:84961859572
SN - 2156-7085
VL - 7
SP - 302
EP - 311
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 2
ER -