Visualization of podocyte substructure with structured illumination microscopy (SIM)

A new approach to nephrotic disease

James M. Pullman, Jonathan Nylk, Elaine C. Campbell, Frank J. Gunn-Moore, Michael B. Prystowsky, Kishan Dholakia

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease.

Original languageEnglish (US)
Pages (from-to)302-311
Number of pages10
JournalBiomedical Optics Express
Volume7
Issue number2
DOIs
StatePublished - Jan 6 2016

Fingerprint

Podocytes
substructures
Lighting
Microscopy
electron microscopy
Electron Microscopy
illumination
microscopy
Biopsy
kidney diseases
glomerulus
Kidney
Kidney Diseases
Frozen Sections
staining
markers
Fluorescent Antibody Technique
Foot
coverings
Staining and Labeling

Keywords

  • Fluorescence microscopy
  • Medical and biological imaging
  • Medical optics and biotechnology
  • Microscopy
  • Optical diagnostics for medicine
  • Optical pathology
  • Superresolution

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Visualization of podocyte substructure with structured illumination microscopy (SIM) : A new approach to nephrotic disease. / Pullman, James M.; Nylk, Jonathan; Campbell, Elaine C.; Gunn-Moore, Frank J.; Prystowsky, Michael B.; Dholakia, Kishan.

In: Biomedical Optics Express, Vol. 7, No. 2, 06.01.2016, p. 302-311.

Research output: Contribution to journalArticle

Pullman, James M. ; Nylk, Jonathan ; Campbell, Elaine C. ; Gunn-Moore, Frank J. ; Prystowsky, Michael B. ; Dholakia, Kishan. / Visualization of podocyte substructure with structured illumination microscopy (SIM) : A new approach to nephrotic disease. In: Biomedical Optics Express. 2016 ; Vol. 7, No. 2. pp. 302-311.
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