In order to gain insight into a possible modulatory role for μ, δ, and κ opioid receptors of the nigrostriatal dopaminergic pathway, we investigated the topographical organization of the receptors with respect to pre- and postsynaptic membranes. Dopaminergic terminals projecting from the substantia nigra to the corpus striatum were destroyed by unilateral injection of 6-hydroxydopamine into the substantia nigra. Quantitative receptor assays using highly specific radioligands were used to measure the density of striatal μ, δ, and κ receptors before and after denervation. Denervation caused a 34 ± 2% loss of striatal μ receptors and a 32 ± 1% loss of striatal δ receptors on the lesioned side of the brain; in contrast, κ receptors did not change significantly in density. Quantitative in vitro autoradiography was used to visualize the neuroanatomical pattern of receptors on lesioned and nonlesioned sides of the brain under the light microscope. Loss of μ receptors in striatal patches was striking in the ventrolateral areas of the striatum, whereas the most notable loss of δ receptors was found in the central striatum. Other brain areas did not differ significantly in μ receptor density between the lesioned and nonlesioned sides, as determined by autoradiography. These findings suggest that a high percentage of μ and δ receptors in the striatum are located on the nigrostriatal dopaminergic terminals and support the concept of a modulatory role for μ and δ opioid peptides in the nigrostriatal dopaminergic pathway.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1987|
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