Visual Snapshots of Intracellular Kinase Activity at the Onset of Mitosis

Zhaohua Dai; Natalya G. Dulyaninova; Sanjai Kumar; Anne R. Bresnick; David S. Lawrence

doi:10.1016/j.chembiol.2007.10.007

Visual Snapshots of Intracellular Kinase Activity at the Onset of Mitosis

Zhaohua Dai, Natalya G. Dulyaninova, Sanjai Kumar, Anne R. Bresnick, David S. Lawrence

Biochemistry

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC β isoform with subsequent NEB.

Original language	English (US)
Pages (from-to)	1254-1260
Number of pages	7
Journal	Chemistry and Biology
Volume	14
Issue number	11
DOIs	https://doi.org/10.1016/j.chembiol.2007.10.007
State	Published - Nov 26 2007

Keywords

CHEMBIO
SIGNALING

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Access to Document

10.1016/j.chembiol.2007.10.007

Cite this

@article{d100cc60161049c2aba6d356e146f88f,

title = "Visual Snapshots of Intracellular Kinase Activity at the Onset of Mitosis",

abstract = "Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC β isoform with subsequent NEB.",

keywords = "CHEMBIO, SIGNALING",

author = "Zhaohua Dai and Dulyaninova, {Natalya G.} and Sanjai Kumar and Bresnick, {Anne R.} and Lawrence, {David S.}",

note = "Funding Information: We thank the National Institutes of Health (CA095019 [D.S.L.] and GM069945 [A.R.B.]) for financial support. D.S.L. is a member of the scientific advisory board of Panomics, the company to which this work has been licensed. ",

year = "2007",

month = nov,

day = "26",

doi = "10.1016/j.chembiol.2007.10.007",

language = "English (US)",

volume = "14",

pages = "1254--1260",

journal = "Chemistry and Biology",

issn = "1074-5521",

publisher = "Elsevier Inc.",

number = "11",

}

TY - JOUR

T1 - Visual Snapshots of Intracellular Kinase Activity at the Onset of Mitosis

AU - Dai, Zhaohua

AU - Dulyaninova, Natalya G.

AU - Kumar, Sanjai

AU - Bresnick, Anne R.

AU - Lawrence, David S.

N1 - Funding Information: We thank the National Institutes of Health (CA095019 [D.S.L.] and GM069945 [A.R.B.]) for financial support. D.S.L. is a member of the scientific advisory board of Panomics, the company to which this work has been licensed.

PY - 2007/11/26

Y1 - 2007/11/26

N2 - Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC β isoform with subsequent NEB.

AB - Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC β isoform with subsequent NEB.

KW - CHEMBIO

KW - SIGNALING

UR - http://www.scopus.com/inward/record.url?scp=36048958871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36048958871&partnerID=8YFLogxK

U2 - 10.1016/j.chembiol.2007.10.007

DO - 10.1016/j.chembiol.2007.10.007

M3 - Article

C2 - 18022564

AN - SCOPUS:36048958871

SN - 1074-5521

VL - 14

SP - 1254

EP - 1260

JO - Chemistry and Biology

JF - Chemistry and Biology

IS - 11

ER -

Visual Snapshots of Intracellular Kinase Activity at the Onset of Mitosis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this