Visual Snapshots of Intracellular Kinase Activity at the Onset of Mitosis

Zhaohua Dai, Natalya G. Dulyaninova, Sanjai Kumar, Anne R. Bresnick, David S. Lawrence

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC β isoform with subsequent NEB.

Original languageEnglish (US)
Pages (from-to)1254-1260
Number of pages7
JournalChemistry and Biology
Volume14
Issue number11
DOIs
StatePublished - Nov 26 2007

Keywords

  • CHEMBIO
  • SIGNALING

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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