TY - JOUR
T1 - Virulence of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis in reconstituted human tissue models
AU - Gácser, Attila
AU - Schäfer, Wilhelm
AU - Nosanchuk, Jerome S.
AU - Salomon, Siegfried
AU - Nosanchuk, Joshua D.
N1 - Funding Information:
We thank Dr. Rani Sellers for useful help concerning histopathological analyses. The authors wish to thank Jozef Nosek, Judit Kucsera, Ilona Pfeiffer for providing the isolates. J.D.N. is supported in part by NIH AI056070-01A2, a Wyeth Vaccine Young Investigator Research Award from the Infectious Disease Society of America, and the Einstein MMC Center for AIDS Research [NIH NIAID AI51519].
PY - 2007/12
Y1 - 2007/12
N2 - Candida parapsilosis is an increasingly important human pathogen. To study the interactions of C. parapsilosis with human tissues, we evaluated the effects of the CBS 604 type strain and three different clinical isolates on reconstituted human oral epithelial and epidermal tissues. The newly described species Candida orthopsilosis and Candida metapsilosis were also examined in these models. Microscopy of reconstituted tissues infected with yeast cells revealed severe attenuation, morphological changes and cellular damage. C. orthopsilosis caused damage similar to C. parapsilosis isolates, whereas C. metapsilosis was less virulent. To further quantitate tissue damage, we measured lactate dehydrogenase (LDH) in the culture supernatant. The relative LDH measurements correlated with our histopathological observations. We also examined the effect of the lipase inhibitor Ebelactone B and proteinase inhibitor Pepstatin A, to establish the utility of this model for studying factors of C. parapsilosis virulence. Both Ebelactone B and Pepstatin A reduced the destruction of epidermal and epithelial tissues. Our data show that reconstituted human tissues are extremely useful for modeling host interactions with C. parapsilosis and for studying fungal virulence factors.
AB - Candida parapsilosis is an increasingly important human pathogen. To study the interactions of C. parapsilosis with human tissues, we evaluated the effects of the CBS 604 type strain and three different clinical isolates on reconstituted human oral epithelial and epidermal tissues. The newly described species Candida orthopsilosis and Candida metapsilosis were also examined in these models. Microscopy of reconstituted tissues infected with yeast cells revealed severe attenuation, morphological changes and cellular damage. C. orthopsilosis caused damage similar to C. parapsilosis isolates, whereas C. metapsilosis was less virulent. To further quantitate tissue damage, we measured lactate dehydrogenase (LDH) in the culture supernatant. The relative LDH measurements correlated with our histopathological observations. We also examined the effect of the lipase inhibitor Ebelactone B and proteinase inhibitor Pepstatin A, to establish the utility of this model for studying factors of C. parapsilosis virulence. Both Ebelactone B and Pepstatin A reduced the destruction of epidermal and epithelial tissues. Our data show that reconstituted human tissues are extremely useful for modeling host interactions with C. parapsilosis and for studying fungal virulence factors.
KW - Candida metapsilosis
KW - Candida orthopsilosis
KW - Candida parapsilosis
KW - Pathogenesis
KW - Reconstituted human tissue
KW - Virulence
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U2 - 10.1016/j.fgb.2007.02.002
DO - 10.1016/j.fgb.2007.02.002
M3 - Article
C2 - 17391997
AN - SCOPUS:34948905295
SN - 1087-1845
VL - 44
SP - 1336
EP - 1341
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
IS - 12
ER -