Virological synapses allow HIV-1 uptake and gene expression in renal tubular epithelial cells

Ping Chen, Benjamin K. Chen, Arevik Mosoian, Thomas Hays, Michael J. Ross, Paul E. Klotman, Mary E. Klotman

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In animal models of HIV-associated nephropathy, the expression of HIV regulatory genes in epithelial cells is sufficient to cause disease, but how the CD4-negative epithelial cells come to express HIV genes is unknown. Here, we co-cultured T cells infected with fluorescently tagged HIV with renal tubular epithelial cells and observed efficient virus transfer between these cells. The quantity of HIV transferred was much greater than that achieved by exposure to large amounts of cell-free virus and occurred without a requirement for CD4 or Env. The transfer required stable cell- cell adhesion, which could be blocked by sulfated polysaccharides or poly-anionic compounds. We found that the internalization of virus could lead to de novo synthesis of viral protein from incoming viral RNAs even in the presence of a reverse transcriptase inhibitor. These results illustrate an interaction between infected T cells and nonimmune cells, supporting the presence of virological synapses between HIV-harboring T cells and renal tubular epithelial cells, allowing viral uptake and gene expression in epithelial cells.

Original languageEnglish (US)
Pages (from-to)496-507
Number of pages12
JournalJournal of the American Society of Nephrology
Volume22
Issue number3
DOIs
StatePublished - Mar 2011
Externally publishedYes

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Synapses
HIV-1
Epithelial Cells
HIV
Kidney
Gene Expression
T-Lymphocytes
AIDS-Associated Nephropathy
Viruses
Virus Internalization
Reverse Transcriptase Inhibitors
Viral Genes
Viral RNA
Viral Proteins
Regulator Genes
Cell Adhesion
Polysaccharides
Cultured Cells
Animal Models
Genes

ASJC Scopus subject areas

  • Nephrology

Cite this

Virological synapses allow HIV-1 uptake and gene expression in renal tubular epithelial cells. / Chen, Ping; Chen, Benjamin K.; Mosoian, Arevik; Hays, Thomas; Ross, Michael J.; Klotman, Paul E.; Klotman, Mary E.

In: Journal of the American Society of Nephrology, Vol. 22, No. 3, 03.2011, p. 496-507.

Research output: Contribution to journalArticle

Chen, Ping ; Chen, Benjamin K. ; Mosoian, Arevik ; Hays, Thomas ; Ross, Michael J. ; Klotman, Paul E. ; Klotman, Mary E. / Virological synapses allow HIV-1 uptake and gene expression in renal tubular epithelial cells. In: Journal of the American Society of Nephrology. 2011 ; Vol. 22, No. 3. pp. 496-507.
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