Virally-directed fluorescent imaging (VFI) can facilitate endoscopic staging

P. S. Adusumilli, D. P. Eisenberg, B. M. Stiles, K. J. Hendershott, S. F. Stanziale, M. K. Chan, M. Hezel, R. Huq, V. W. Rusch, Y. Fong

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Background: Replication-competent, tumor specific herpes simplex virus NV1066 expresses green fluorescent protein (GFP) in infected cancer cells. We sought to determine the feasibility of GFP-guided imaging technology in the intraoperative detection of small tumor nodules. Methods: Human cancer cell lines were infected with NV1066 at multiplicities of infection of 0.01, 0.1 and 1. Cancer cell specific infectivity, vector spread and GFP signal intensity were measured by flow cytometry and time-lapse digital imaging (in vitro); and by use of a stereomicroscope and endoscope equipped with a fluorescent filter (in vivo). Results: NV1066 infected all cancer cell lines and expressed GFP at all MOIs. GFP signal was significantly higher than the autofluorescence of normal cells. One single dose of NV1066 spread within and across body cavities and selectively infected tumor nodules sparing normal tissue. Tumor nodules undetectable by conventional thoracoscopy and laparoscopy were identified by GFP fluorescence. Conclusion: Virally-directed fluorescent imaging (VFI) is a real-time novel molecular imaging technology that has the potential to enhance the intraoperative detection of endoluminal or endocavitary tumor nodules.

Original languageEnglish (US)
Pages (from-to)628-635
Number of pages8
JournalSurgical Endoscopy and Other Interventional Techniques
Volume20
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Keywords

  • Fluorescent detection
  • Fluorescent laparoscopy
  • Fluorescent thoracoscopy
  • Gene therapy
  • Herpes simplex virus
  • Oncolytic virus

ASJC Scopus subject areas

  • Surgery

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