Viral delivery of GFP-dependent recombinases to the mouse brain

Jonathan C.Y. Tang, Stephanie Rudolph, Constance L. Cepko

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Many genetic tools have been developed that use green fluorescent protein (GFP) and its derivatives for labeling specific cell populations in organisms and in cell culture. To extend the use of GFP beyond labeling purposes, we developed methods and reagents that use GFP as a driver of biological activities. We used nanobodies that bind GFP to engineer CRE-DOG and Flp-DOG, recombinases that can induce Cre/lox and Flp/FRT recombination in a GFP-dependent manner, respectively. Here, we present a protocol to deliver CRE-DOG and Flp-DOG into the mouse brain by recombinant AAV infection. This protocol enables one to manipulate gene expression specifically in GFP-expressing cells, found either in transgenic GFP reporter lines or in cells made to express GFP by other transduction methods.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages109-126
Number of pages18
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1642
ISSN (Print)1064-3745

Keywords

  • Adeno-associated viruses
  • Cell type-specific manipulation
  • Green fluorescent protein
  • Transgenic mouse

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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