Vesicular transport in Histoplasma capsulatum

An effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes

Priscila Costa Albuquerque, Ernesto S. Nakayasu, Marcio L. Rodrigues, Susana Frases, Arturo Casadevall, Rosely M. Zancope-Oliveira, Igor C. Almeida, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Vesicular secretion of macromolecules has recently been described in the basidiomycete Cryptococcus neoformans, raising the question as to whether ascomycetes similarly utilize vesicles for transport. In the present study, we examine whether the clinically important ascomycete Histoplasma capsulatum produce vesicles and utilized these structures to secrete macromolecules. Transmission electron microscopy (TEM) shows transcellular secretion of vesicles by yeast cells. Proteomic and lipidomic analyses of vesicles isolated from culture supernatants reveal a rich collection of macromolecules involved in diverse processes, including metabolism, cell recycling, signalling and virulence. The results demonstrate that H. capsulatum can utilize a trans-cell wall vesicular transport secretory mechanism to promote virulence. Additionally, TEM of supernatants collected from Candida albicans, Candida parapsilosis, Sporothrix schenckii and Saccharomyces cerevisiae documents that vesicles are similarly produced by additional ascomycetes. The vesicles from H. capsulatum react with immune serum from patients with histoplasmosis, providing an association of the vesicular products with pathogenesis. The findings support the proposal that vesicular secretion is a general mechanism in fungi for the transport of macromolecules related to virulence and that this process could be a target for novel therapeutics.

Original languageEnglish (US)
Pages (from-to)1695-1710
Number of pages16
JournalCellular Microbiology
Volume10
Issue number8
DOIs
StatePublished - Aug 2008

Fingerprint

Histoplasma
Ascomycota
Macromolecules
Cell Wall
Virulence
Cells
Transmission Electron Microscopy
Candida
Sporothrix
Yeast
Transport Vesicles
Histoplasmosis
Basidiomycota
Cryptococcus neoformans
Transmission electron microscopy
Candida albicans
Proteomics
Saccharomyces cerevisiae
Immune Sera
Fungi

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

Vesicular transport in Histoplasma capsulatum : An effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. / Albuquerque, Priscila Costa; Nakayasu, Ernesto S.; Rodrigues, Marcio L.; Frases, Susana; Casadevall, Arturo; Zancope-Oliveira, Rosely M.; Almeida, Igor C.; Nosanchuk, Joshua D.

In: Cellular Microbiology, Vol. 10, No. 8, 08.2008, p. 1695-1710.

Research output: Contribution to journalArticle

Albuquerque, PC, Nakayasu, ES, Rodrigues, ML, Frases, S, Casadevall, A, Zancope-Oliveira, RM, Almeida, IC & Nosanchuk, JD 2008, 'Vesicular transport in Histoplasma capsulatum: An effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes', Cellular Microbiology, vol. 10, no. 8, pp. 1695-1710. https://doi.org/10.1111/j.1462-5822.2008.01160.x
Albuquerque, Priscila Costa ; Nakayasu, Ernesto S. ; Rodrigues, Marcio L. ; Frases, Susana ; Casadevall, Arturo ; Zancope-Oliveira, Rosely M. ; Almeida, Igor C. ; Nosanchuk, Joshua D. / Vesicular transport in Histoplasma capsulatum : An effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. In: Cellular Microbiology. 2008 ; Vol. 10, No. 8. pp. 1695-1710.
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