Vesicular trans-cell wall Transport in fungi: A mechanism for the delivery of virulence-associated macromolecules?

Marcio L. Rodrigues, Leonardo Nimrichter, Debora L. Oliveira, Joshua D. Nosanchuk, Arturo Casadevall

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Fungal cells are encaged in rigid, complex cell walls. Until recently, there was remarkably little information regarding the trans-fungal cell wall transfer of intracellular macromolecules to the extracellular space. Recently, several studies have begun to elucidate the mechanisms that fungal cells utilize to secrete a wide variety of macromolecules through the cell wall. The combined use of transmission electron microscopy, serology, biochemistry, proteomics and lipidomics have revealed that the fungal pathogens Cryptococcus neoformans, Histoplasma capsulatum, Candida albicans, Candida parapsilosis and Sporothrix schenckii, as well as the model yeast Saccharomyces cerevisiae, each produces extracellular vesicles that carry lipids, proteins, polysaccharides and pigment-like structures of unquestionable biological significance. Compositional analysis of the C. neoformans and H. capsulatum extracellular vesicles suggests that they may function as 'virulence bags', with the potential to modulate the host-pathogen interaction in favor of the fungus. The cellular origin of the extracellular vesicles remains unknown, but morphological and biochemical features indicate that they are similar to the well-described mammalian exosomes.

Original languageEnglish (US)
Pages (from-to)27-40
Number of pages14
JournalLipid Insights
Volume2 1
StatePublished - 2008

Fingerprint

Fungi
Macromolecules
Cell Wall
Histoplasma
Virulence
Cryptococcus neoformans
Candida
Cells
Pathogens
Yeast
Sporothrix
Exosomes
Host-Pathogen Interactions
Biochemistry
Extracellular Space
Serology
Candida albicans
Transmission Electron Microscopy
Pigments
Proteomics

Keywords

  • Extracellular vesicles
  • Secretion
  • Trans-cell wall transport

ASJC Scopus subject areas

  • Biochemistry

Cite this

Vesicular trans-cell wall Transport in fungi : A mechanism for the delivery of virulence-associated macromolecules? / Rodrigues, Marcio L.; Nimrichter, Leonardo; Oliveira, Debora L.; Nosanchuk, Joshua D.; Casadevall, Arturo.

In: Lipid Insights, Vol. 2 1, 2008, p. 27-40.

Research output: Contribution to journalArticle

Rodrigues, Marcio L. ; Nimrichter, Leonardo ; Oliveira, Debora L. ; Nosanchuk, Joshua D. ; Casadevall, Arturo. / Vesicular trans-cell wall Transport in fungi : A mechanism for the delivery of virulence-associated macromolecules?. In: Lipid Insights. 2008 ; Vol. 2 1. pp. 27-40.
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