Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport

Marcio L. Rodrigues, Leonardo Nimrichter, Débora L. Oliveira, Susana Frases, Kildare Miranda, Oscar Zaragoza, Mauricio Alvarez, Antonio Nakouzi, Marta Feldmesser, Arturo Casadevall

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

The mechanisms by which macromolecules are transported through the cell wall of fungi are not known. A central question in the biology of Cryptococcus neoformans, the causative agent of cryptococcosis, is the mechanism by which capsular polysaccharide synthesized inside the cell is exported to the extracellular environment for capsule assembly and release. We demonstrate that C. neoformans produces extracellular vesicles during in vitro growth and animal infection. Vesicular compartments, which are transferred to the extracellular space by cell wall passage, contain glucuronoxylomannan (GXM), a component of the cryptococcal capsule, and key lipids, such as glucosylceramide and sterols. A correlation between GXM-containing vesicles and capsule expression was observed. The results imply a novel mechanism for the release of the major virulence factor of C. neoformans whereby polysaccharide packaged in lipid vesicles crosses the cell wall and the capsule network to reach the extracellular environment.

Original languageEnglish (US)
Pages (from-to)48-59
Number of pages12
JournalEukaryotic Cell
Volume6
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Cryptococcus neoformans
Cell Wall
Capsules
Polysaccharides
polysaccharides
cell walls
extracellular space
lipids
Glucosylceramides
Lipids
Cryptococcosis
sterols
Extracellular Space
Virulence Factors
Sterols
virulence
Biological Sciences
fungi
Fungi
infection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Rodrigues, M. L., Nimrichter, L., Oliveira, D. L., Frases, S., Miranda, K., Zaragoza, O., ... Casadevall, A. (2007). Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport. Eukaryotic Cell, 6(1), 48-59. https://doi.org/10.1128/EC.00318-06

Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport. / Rodrigues, Marcio L.; Nimrichter, Leonardo; Oliveira, Débora L.; Frases, Susana; Miranda, Kildare; Zaragoza, Oscar; Alvarez, Mauricio; Nakouzi, Antonio; Feldmesser, Marta; Casadevall, Arturo.

In: Eukaryotic Cell, Vol. 6, No. 1, 01.2007, p. 48-59.

Research output: Contribution to journalArticle

Rodrigues, ML, Nimrichter, L, Oliveira, DL, Frases, S, Miranda, K, Zaragoza, O, Alvarez, M, Nakouzi, A, Feldmesser, M & Casadevall, A 2007, 'Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport', Eukaryotic Cell, vol. 6, no. 1, pp. 48-59. https://doi.org/10.1128/EC.00318-06
Rodrigues, Marcio L. ; Nimrichter, Leonardo ; Oliveira, Débora L. ; Frases, Susana ; Miranda, Kildare ; Zaragoza, Oscar ; Alvarez, Mauricio ; Nakouzi, Antonio ; Feldmesser, Marta ; Casadevall, Arturo. / Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport. In: Eukaryotic Cell. 2007 ; Vol. 6, No. 1. pp. 48-59.
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