Ventral tegmental microinjection of Δ9-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient

Jianping Chen, Ronen Marmur, Addy Pulles, William Paredes, Eliot L. Gardner

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Basal extracellular levels of dopamine (DA) and its metabolites in both ventral tegmental area (VTA) and nucleus accumbens (Acb) were simultaneously monitored by in vivo brain microdialysis in laboratory rats. Microinjection of 12 μg or 24 μg Δ9-tetrahydrocannabinol (Δ9-THC), the psychoactive ingredient in marijuana and hashish, into the VTA produced a dose-dependent increase in somatodendritic DA levels in VTA but failed to produce any simulatenous change in extracellular DA in Acb. Direct Δ9-THC perfusion (5×10-5 and 2×10-4 M) into Acb via the microdialysis probes caused a significant increase in extracellular DA levels in Acb. These findings suggest that (1) Δ9-THC has a facilitating effect on somatodendritic DA efflux, and (2) the elevation of Acb DA levels seen in our previous studies with systemic administration of Δ9-THC may result from local actions of Δ9-THC at or near the DA terminal projections in Acb.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalBrain Research
Volume621
Issue number1
DOIs
StatePublished - Sep 3 1993

Fingerprint

Dronabinol
Microinjections
Cannabis
Prosencephalon
Dopamine
Ventral Tegmental Area
Microdialysis
Tegmentum Mesencephali
Nucleus Accumbens
Perfusion
Brain

Keywords

  • Addiction
  • Brain reward
  • Cannabinoid
  • Cannabis
  • Dopamine
  • Marijuana
  • Microdialysis
  • Nucleus accumbens
  • Ventral tegmental area
  • Δ-Tetrahydrocannabinol (Δ-THC)

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

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title = "Ventral tegmental microinjection of Δ9-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels: evidence for local neural action by marijuana's psychoactive ingredient",
abstract = "Basal extracellular levels of dopamine (DA) and its metabolites in both ventral tegmental area (VTA) and nucleus accumbens (Acb) were simultaneously monitored by in vivo brain microdialysis in laboratory rats. Microinjection of 12 μg or 24 μg Δ9-tetrahydrocannabinol (Δ9-THC), the psychoactive ingredient in marijuana and hashish, into the VTA produced a dose-dependent increase in somatodendritic DA levels in VTA but failed to produce any simulatenous change in extracellular DA in Acb. Direct Δ9-THC perfusion (5×10-5 and 2×10-4 M) into Acb via the microdialysis probes caused a significant increase in extracellular DA levels in Acb. These findings suggest that (1) Δ9-THC has a facilitating effect on somatodendritic DA efflux, and (2) the elevation of Acb DA levels seen in our previous studies with systemic administration of Δ9-THC may result from local actions of Δ9-THC at or near the DA terminal projections in Acb.",
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T1 - Ventral tegmental microinjection of Δ9-tetrahydrocannabinol enhances ventral tegmental somatodendritic dopamine levels but not forebrain dopamine levels

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AU - Marmur, Ronen

AU - Pulles, Addy

AU - Paredes, William

AU - Gardner, Eliot L.

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N2 - Basal extracellular levels of dopamine (DA) and its metabolites in both ventral tegmental area (VTA) and nucleus accumbens (Acb) were simultaneously monitored by in vivo brain microdialysis in laboratory rats. Microinjection of 12 μg or 24 μg Δ9-tetrahydrocannabinol (Δ9-THC), the psychoactive ingredient in marijuana and hashish, into the VTA produced a dose-dependent increase in somatodendritic DA levels in VTA but failed to produce any simulatenous change in extracellular DA in Acb. Direct Δ9-THC perfusion (5×10-5 and 2×10-4 M) into Acb via the microdialysis probes caused a significant increase in extracellular DA levels in Acb. These findings suggest that (1) Δ9-THC has a facilitating effect on somatodendritic DA efflux, and (2) the elevation of Acb DA levels seen in our previous studies with systemic administration of Δ9-THC may result from local actions of Δ9-THC at or near the DA terminal projections in Acb.

AB - Basal extracellular levels of dopamine (DA) and its metabolites in both ventral tegmental area (VTA) and nucleus accumbens (Acb) were simultaneously monitored by in vivo brain microdialysis in laboratory rats. Microinjection of 12 μg or 24 μg Δ9-tetrahydrocannabinol (Δ9-THC), the psychoactive ingredient in marijuana and hashish, into the VTA produced a dose-dependent increase in somatodendritic DA levels in VTA but failed to produce any simulatenous change in extracellular DA in Acb. Direct Δ9-THC perfusion (5×10-5 and 2×10-4 M) into Acb via the microdialysis probes caused a significant increase in extracellular DA levels in Acb. These findings suggest that (1) Δ9-THC has a facilitating effect on somatodendritic DA efflux, and (2) the elevation of Acb DA levels seen in our previous studies with systemic administration of Δ9-THC may result from local actions of Δ9-THC at or near the DA terminal projections in Acb.

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