Vascular superoxide and hydrogen peroxide production and oxidative stress resistance in two closely related rodent species with disparate longevity

Anna Csiszar, Nazar Labinskyy, Xiangmin Zhao, Furong Hu, Sabrina Serpillon, Zhishan Huang, Praveen Ballabh, Richard J. Levy, Thomas H. Hintze, Michael S. Wolin, Steven N. Austad, Andrej Podlutsky, Zoltan Ungvari

Research output: Contribution to journalArticle

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Abstract

Vascular aging is characterized by increased oxidative stress, impaired nitric oxide (NO) bioavailability and enhanced apoptotic cell death. The oxidative stress hypothesis of aging predicts that vascular cells of long-lived species exhibit lower production of reactive oxygen species (ROS) and/or superior resistance to oxidative stress. We tested this hypothesis using two taxonomically related rodents, the white-footed mouse (Peromyscus leucopus) and the house mouse (Mus musculus), that show a more than twofold difference in maximum lifespan potential (MLSP = 8 and 3.5 years, respectively). We compared interspecies differences in endothelial superoxide (O2) and hydrogen peroxide (H2O2) production, NAD(P)H oxidase activity, mitochondrial ROS generation, expression of pro- and antioxidant enzymes, NO production, and resistance to oxidative stress-induced apoptosis. In aortas of P. leucopus, NAD(P)H oxidase expression and activity, endothelial and H2O2 production, and ROS generation by mitochondria were less than in mouse vessels. In P. leucopus , there was a more abundant expression of catalase, glutathione peroxidase 1 and hemeoxygenase-1, whereas expression of Cu/Zn-SOD and Mn-SOD was similar in both species. NO production and endothelial nitric oxide synthase expression was greater in P. leucopus . In mouse aortas, treatment with oxidized low-density lipoprotein (oxLDL) elicited substantial oxidative stress, endothelial dysfunction and endothelial apoptosis (assessed by TUNEL assay, DNA fragmentation and caspase 3 activity assays). According to our prediction, vessels of P. leucopus were more resistant to the proapoptotic effects of oxidative stressors (oxLDL and H2O2). Primary fibroblasts from P. leucopus also exhibited less H2O2-induced DNA damage (comet assay) than mouse cells. Thus, increased lifespan potential in P. leucopus is associated with a decreased cellular ROS generation and increased oxidative stress resistance, which accords with the prediction of the oxidative stress hypothesis of aging.

Original languageEnglish (US)
Pages (from-to)783-797
Number of pages15
JournalAging Cell
Volume6
Issue number6
DOIs
StatePublished - Dec 2007
Externally publishedYes

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Superoxides
Hydrogen Peroxide
Blood Vessels
Rodentia
Oxidative Stress
Reactive Oxygen Species
Peromyscus
Nitric Oxide
NADPH Oxidase
Aorta
Apoptosis
Heme Oxygenase-1
Comet Assay
Nitric Oxide Synthase Type III
In Situ Nick-End Labeling
DNA Fragmentation
Caspase 3
Catalase
Biological Availability
DNA Damage

Keywords

  • Atherosclerosis
  • Comparative biology
  • Oxidative stress
  • Sene scence
  • Vascular disease

ASJC Scopus subject areas

  • Cell Biology

Cite this

Vascular superoxide and hydrogen peroxide production and oxidative stress resistance in two closely related rodent species with disparate longevity. / Csiszar, Anna; Labinskyy, Nazar; Zhao, Xiangmin; Hu, Furong; Serpillon, Sabrina; Huang, Zhishan; Ballabh, Praveen; Levy, Richard J.; Hintze, Thomas H.; Wolin, Michael S.; Austad, Steven N.; Podlutsky, Andrej; Ungvari, Zoltan.

In: Aging Cell, Vol. 6, No. 6, 12.2007, p. 783-797.

Research output: Contribution to journalArticle

Csiszar, A, Labinskyy, N, Zhao, X, Hu, F, Serpillon, S, Huang, Z, Ballabh, P, Levy, RJ, Hintze, TH, Wolin, MS, Austad, SN, Podlutsky, A & Ungvari, Z 2007, 'Vascular superoxide and hydrogen peroxide production and oxidative stress resistance in two closely related rodent species with disparate longevity', Aging Cell, vol. 6, no. 6, pp. 783-797. https://doi.org/10.1111/j.1474-9726.2007.00339.x
Csiszar, Anna ; Labinskyy, Nazar ; Zhao, Xiangmin ; Hu, Furong ; Serpillon, Sabrina ; Huang, Zhishan ; Ballabh, Praveen ; Levy, Richard J. ; Hintze, Thomas H. ; Wolin, Michael S. ; Austad, Steven N. ; Podlutsky, Andrej ; Ungvari, Zoltan. / Vascular superoxide and hydrogen peroxide production and oxidative stress resistance in two closely related rodent species with disparate longevity. In: Aging Cell. 2007 ; Vol. 6, No. 6. pp. 783-797.
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AU - Serpillon, Sabrina

AU - Huang, Zhishan

AU - Ballabh, Praveen

AU - Levy, Richard J.

AU - Hintze, Thomas H.

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