Vascular responses to angiotensin-(1-7) during the estrous cycle

Liomar A A Neves, David B. Averill, Carlos M. Ferrario, Judy L. Aschner, K. Bridget Brosnihan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mesenteric arteries (230-290 μm) were isolated from virgin female rats at diestrous and proestrous phases of the estrous cycle and from ovariectomized (OVX) rats with or without estrogen (E 2) replacement (17β-estradiol, 7.5 + 5 mg pellets, 21 d release). Arteries were mounted in a pressurized myograph system. Angiotensin-(1-7) [Ang-(1-7)] concentration-dependent responses (10 -10-10 -5 M) were determined in arteries preconstricted with endothelin-1 (10 -7 M). Mesenteric arteries were pretreated with the specific Ang-(1-7) antagonist, D-[Ala 7]-Ang-(1-7) (10 -7 M) to assess the Ang-(1-7) receptor-mediated dilator effect. Ang-(1-7) did not dilate mesenteric arteries from virgin rats at diestrus and placebo-treated OVX female rats as compared to the time control; however, Ang-(1-7) elicited a modest dilation at proestrus as compared to diestrus, which reached statistical significance at 10 -8 M concentrations. Ang-(1-7) caused a concentration-dependent vasodilation in mesenteric arteries of females with E 2 replacement, with an EC 50 of 21 nM. D-[Ala 7]-Ang-(1-7) blocked the vasodilator effect of Ang-(1-7). Our results demonstrate that during proestrus Ang-(1-7) elicits modest vasodilation as compared to diestrus, but lacks vasodilatory properties in vessels from diestrous and ovariectomized rats. Estrogen replacement restores a significant dilator response to Ang-(1-7) in OVX rats that is mediated by a D-[Ala 7]-Ang-(1-7) sensitive site.

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalEndocrine
Volume24
Issue number2
DOIs
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Estrous Cycle
Blood Vessels
7-Ala-angiotensin (1-7)
Mesenteric Arteries
Diestrus
Proestrus
Vasodilation
Arteries
angiotensin I (1-7)
Estrogen Replacement Therapy
Endothelin-1
Vasodilator Agents
Dilatation
Estradiol
Estrogens
Placebos

Keywords

  • Estrogen replacement
  • Hypertension
  • Renin-angiotensin system
  • Vasodilation

ASJC Scopus subject areas

  • Endocrinology

Cite this

Neves, L. A. A., Averill, D. B., Ferrario, C. M., Aschner, J. L., & Brosnihan, K. B. (2004). Vascular responses to angiotensin-(1-7) during the estrous cycle. Endocrine, 24(2), 161-165. https://doi.org/10.1385/ENDO:24:2:161

Vascular responses to angiotensin-(1-7) during the estrous cycle. / Neves, Liomar A A; Averill, David B.; Ferrario, Carlos M.; Aschner, Judy L.; Brosnihan, K. Bridget.

In: Endocrine, Vol. 24, No. 2, 07.2004, p. 161-165.

Research output: Contribution to journalArticle

Neves, LAA, Averill, DB, Ferrario, CM, Aschner, JL & Brosnihan, KB 2004, 'Vascular responses to angiotensin-(1-7) during the estrous cycle', Endocrine, vol. 24, no. 2, pp. 161-165. https://doi.org/10.1385/ENDO:24:2:161
Neves, Liomar A A ; Averill, David B. ; Ferrario, Carlos M. ; Aschner, Judy L. ; Brosnihan, K. Bridget. / Vascular responses to angiotensin-(1-7) during the estrous cycle. In: Endocrine. 2004 ; Vol. 24, No. 2. pp. 161-165.
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