Vascular mural cells in healing canine myocardial infarcts

Marcin Dobaczewski, Spyridon Akrivakis, Kamal Nasser, Lloyd H. Michael, Mark L. Entman, Nikolaos G. Frangogiannis

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Angiogenesis is a critical process in healing of myocardial infarcts, leading to the formation of highly vascular granulation tissue. However, effective cardiac repair depends on mechanisms that inhibit the angiogenic process after a mature scar is formed, preventing inappropriate expansion of the fibrotic process. Using a canine model of reperfused myocardial infarction, we demonstrated that maturation of the infarct leads to the formation of neovessels, with a thick muscular coat, that demonstrate distinct morphological characteristics. Many of these "neoarterioles" lack a defined internal elastic lamina and demonstrate irregular deposits of extracellular matrix in the media. Vascular mural cells in healing infarcts undergo phenotypic changes, showing minimal expression of desmin during the proliferative phase (1 hr occlusion/7 days reperfusion) but in the mature scar (8 weeks reperfusion) acquire a phenotype similar to that of vascular smooth muscle cells in control areas. Non-muscle myosin heavy chains A and B are induced in infarct endothelial cells and myofibroblasts, respectively, but are not expressed in neovascular mural cells. Recruitment of a muscular coat and formation of neoarterioles in mature scars may inhibit endothelial cell proliferation and vascular sprouting, stabilizing the infarct vasculature.

Original languageEnglish (US)
Pages (from-to)1019-1029
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume52
Issue number8
DOIs
StatePublished - Aug 2004
Externally publishedYes

Fingerprint

Cicatrix
Blood Vessels
Canidae
Myocardial Infarction
Reperfusion
Endothelial Cells
Desmin
Myofibroblasts
Granulation Tissue
Vascular Smooth Muscle
Smooth Muscle Myocytes
Extracellular Matrix
Cell Proliferation
Phenotype
nonmuscle myosin type IIB heavy chain

Keywords

  • α-smooth muscle actin
  • Angiogenesis
  • Arteriole
  • Chain
  • Desmin
  • Myocardial infarction
  • Myofibroblast
  • Non-muscle myosin heavy
  • Pericyte
  • Smooth muscle cell
  • Smoothelin

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Vascular mural cells in healing canine myocardial infarcts. / Dobaczewski, Marcin; Akrivakis, Spyridon; Nasser, Kamal; Michael, Lloyd H.; Entman, Mark L.; Frangogiannis, Nikolaos G.

In: Journal of Histochemistry and Cytochemistry, Vol. 52, No. 8, 08.2004, p. 1019-1029.

Research output: Contribution to journalArticle

Dobaczewski, M, Akrivakis, S, Nasser, K, Michael, LH, Entman, ML & Frangogiannis, NG 2004, 'Vascular mural cells in healing canine myocardial infarcts', Journal of Histochemistry and Cytochemistry, vol. 52, no. 8, pp. 1019-1029. https://doi.org/10.1369/jhc.3A6210.2004
Dobaczewski, Marcin ; Akrivakis, Spyridon ; Nasser, Kamal ; Michael, Lloyd H. ; Entman, Mark L. ; Frangogiannis, Nikolaos G. / Vascular mural cells in healing canine myocardial infarcts. In: Journal of Histochemistry and Cytochemistry. 2004 ; Vol. 52, No. 8. pp. 1019-1029.
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