Vascular endothelial growth factor in dialysate in relation to intensity of peritoneal inflammation

K. Pawlaczyk, A. Polubinska, M. Numata, M. Nakayama, R. Pecoits-Filho, S. Czekalski, Bengt Lindholm, A. Breborowicz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Peritoneal inflammation may induce changes in peritoneal microvessels, including neoangiogenesis/vasculogenesis, leading to increased peritoneal solute transport rate (PSTR) and loss of ultrafiltration capacity. We hypothesized that an inflammatory reaction in the peritoneal cavity during peritonitis induces increased synthesis of vascular endothelial growth factor (VEGF). We therefore studied the relationship between peritoneal inflammation markers, VEGF, and transport of fluid and solutes in rats during acute peritoneal inflammation induced by lipopolysaccharide (LPS) added to standard glucose-based dialysis solution. Methods: Under ether anesthesia, male Wistar rats were injected intraperitoneally with 30 mL Dianeal 3.86% without (Control; n=6) or with LPS (μg/mL): 0.001 (LPS 0.001; n=6), 0.01 (LPS 0.01; n=7), 0.1 (LPS 0.1; n=7), 1.0 (LPS 1.0; n=8). After 8 hours, dialysate volume (IPV), peritoneal solute transport rate (PSTR) and dialysate cell count (DCC) were measured and effluent samples were collected. Results: LPS i.p. resulted in increased PSTR and decreased IPV (p<0.005). DCC (cells/μL) and the neutrophil/macrophage ratio were higher for all LPS concentrations compared to the control group. After 8 hours, LPS-exposed rats had significantly higher dialysate levels of all investigated cytokines (TNF-α, MCP-1 and IL-10) than the control group. Addition of LPS resulted in increased dialysate VEGF concentrations (pg/mL) (LPS 0.001, 28.2±5.9; LPS 0.01, 38.9±11.6; LPS 0.1, 43.0±5.9; LPS 1.0, 46.6±11.3; Control, 14.5±9.8; p<0.0005 for all LPS vs. Control). Conclusions: The infusion of Dianeal 3.86% with different doses of LPS induced a strong acute intraperitoneal inflammatory reaction with increased DCC and cytokine levels, resulting in increased peritoneal solute transport and decreased IPV. LPS induced a dose-dependent parallel increase of the intraperitoneal concentrations of MCP-1, IL-10 and TNF-α, as well as of VEGF. These results suggest that intraperitoneal VEGF synthesis is induced in response to inflammation, and that this may be an important component in the process leading to peritoneal transport alterations.

Original languageEnglish (US)
Pages (from-to)535-544
Number of pages10
JournalInternational Journal of Artificial Organs
Volume31
Issue number6
StatePublished - Jun 2008
Externally publishedYes

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Dialysis Solutions
Solute transport
Vascular Endothelial Growth Factor A
Lipopolysaccharides
Inflammation
Rats
Dialysis
Macrophages
Ultrafiltration
Glucose
Effluents
Ethers
Intercellular Signaling Peptides and Proteins
Cell Count
Fluids
Interleukin-10
Cytokines
Control Groups
Peritoneal Cavity
Microvessels

Keywords

  • Animal model
  • Cytokines
  • Lipopolysaccharide
  • Peritoneal dialysis
  • Peritoneal inflammation
  • Peritoneal transport
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Biophysics

Cite this

Pawlaczyk, K., Polubinska, A., Numata, M., Nakayama, M., Pecoits-Filho, R., Czekalski, S., ... Breborowicz, A. (2008). Vascular endothelial growth factor in dialysate in relation to intensity of peritoneal inflammation. International Journal of Artificial Organs, 31(6), 535-544.

Vascular endothelial growth factor in dialysate in relation to intensity of peritoneal inflammation. / Pawlaczyk, K.; Polubinska, A.; Numata, M.; Nakayama, M.; Pecoits-Filho, R.; Czekalski, S.; Lindholm, Bengt; Breborowicz, A.

In: International Journal of Artificial Organs, Vol. 31, No. 6, 06.2008, p. 535-544.

Research output: Contribution to journalArticle

Pawlaczyk, K, Polubinska, A, Numata, M, Nakayama, M, Pecoits-Filho, R, Czekalski, S, Lindholm, B & Breborowicz, A 2008, 'Vascular endothelial growth factor in dialysate in relation to intensity of peritoneal inflammation', International Journal of Artificial Organs, vol. 31, no. 6, pp. 535-544.
Pawlaczyk K, Polubinska A, Numata M, Nakayama M, Pecoits-Filho R, Czekalski S et al. Vascular endothelial growth factor in dialysate in relation to intensity of peritoneal inflammation. International Journal of Artificial Organs. 2008 Jun;31(6):535-544.
Pawlaczyk, K. ; Polubinska, A. ; Numata, M. ; Nakayama, M. ; Pecoits-Filho, R. ; Czekalski, S. ; Lindholm, Bengt ; Breborowicz, A. / Vascular endothelial growth factor in dialysate in relation to intensity of peritoneal inflammation. In: International Journal of Artificial Organs. 2008 ; Vol. 31, No. 6. pp. 535-544.
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abstract = "Background: Peritoneal inflammation may induce changes in peritoneal microvessels, including neoangiogenesis/vasculogenesis, leading to increased peritoneal solute transport rate (PSTR) and loss of ultrafiltration capacity. We hypothesized that an inflammatory reaction in the peritoneal cavity during peritonitis induces increased synthesis of vascular endothelial growth factor (VEGF). We therefore studied the relationship between peritoneal inflammation markers, VEGF, and transport of fluid and solutes in rats during acute peritoneal inflammation induced by lipopolysaccharide (LPS) added to standard glucose-based dialysis solution. Methods: Under ether anesthesia, male Wistar rats were injected intraperitoneally with 30 mL Dianeal 3.86{\%} without (Control; n=6) or with LPS (μg/mL): 0.001 (LPS 0.001; n=6), 0.01 (LPS 0.01; n=7), 0.1 (LPS 0.1; n=7), 1.0 (LPS 1.0; n=8). After 8 hours, dialysate volume (IPV), peritoneal solute transport rate (PSTR) and dialysate cell count (DCC) were measured and effluent samples were collected. Results: LPS i.p. resulted in increased PSTR and decreased IPV (p<0.005). DCC (cells/μL) and the neutrophil/macrophage ratio were higher for all LPS concentrations compared to the control group. After 8 hours, LPS-exposed rats had significantly higher dialysate levels of all investigated cytokines (TNF-α, MCP-1 and IL-10) than the control group. Addition of LPS resulted in increased dialysate VEGF concentrations (pg/mL) (LPS 0.001, 28.2±5.9; LPS 0.01, 38.9±11.6; LPS 0.1, 43.0±5.9; LPS 1.0, 46.6±11.3; Control, 14.5±9.8; p<0.0005 for all LPS vs. Control). Conclusions: The infusion of Dianeal 3.86{\%} with different doses of LPS induced a strong acute intraperitoneal inflammatory reaction with increased DCC and cytokine levels, resulting in increased peritoneal solute transport and decreased IPV. LPS induced a dose-dependent parallel increase of the intraperitoneal concentrations of MCP-1, IL-10 and TNF-α, as well as of VEGF. These results suggest that intraperitoneal VEGF synthesis is induced in response to inflammation, and that this may be an important component in the process leading to peritoneal transport alterations.",
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T1 - Vascular endothelial growth factor in dialysate in relation to intensity of peritoneal inflammation

AU - Pawlaczyk, K.

AU - Polubinska, A.

AU - Numata, M.

AU - Nakayama, M.

AU - Pecoits-Filho, R.

AU - Czekalski, S.

AU - Lindholm, Bengt

AU - Breborowicz, A.

PY - 2008/6

Y1 - 2008/6

N2 - Background: Peritoneal inflammation may induce changes in peritoneal microvessels, including neoangiogenesis/vasculogenesis, leading to increased peritoneal solute transport rate (PSTR) and loss of ultrafiltration capacity. We hypothesized that an inflammatory reaction in the peritoneal cavity during peritonitis induces increased synthesis of vascular endothelial growth factor (VEGF). We therefore studied the relationship between peritoneal inflammation markers, VEGF, and transport of fluid and solutes in rats during acute peritoneal inflammation induced by lipopolysaccharide (LPS) added to standard glucose-based dialysis solution. Methods: Under ether anesthesia, male Wistar rats were injected intraperitoneally with 30 mL Dianeal 3.86% without (Control; n=6) or with LPS (μg/mL): 0.001 (LPS 0.001; n=6), 0.01 (LPS 0.01; n=7), 0.1 (LPS 0.1; n=7), 1.0 (LPS 1.0; n=8). After 8 hours, dialysate volume (IPV), peritoneal solute transport rate (PSTR) and dialysate cell count (DCC) were measured and effluent samples were collected. Results: LPS i.p. resulted in increased PSTR and decreased IPV (p<0.005). DCC (cells/μL) and the neutrophil/macrophage ratio were higher for all LPS concentrations compared to the control group. After 8 hours, LPS-exposed rats had significantly higher dialysate levels of all investigated cytokines (TNF-α, MCP-1 and IL-10) than the control group. Addition of LPS resulted in increased dialysate VEGF concentrations (pg/mL) (LPS 0.001, 28.2±5.9; LPS 0.01, 38.9±11.6; LPS 0.1, 43.0±5.9; LPS 1.0, 46.6±11.3; Control, 14.5±9.8; p<0.0005 for all LPS vs. Control). Conclusions: The infusion of Dianeal 3.86% with different doses of LPS induced a strong acute intraperitoneal inflammatory reaction with increased DCC and cytokine levels, resulting in increased peritoneal solute transport and decreased IPV. LPS induced a dose-dependent parallel increase of the intraperitoneal concentrations of MCP-1, IL-10 and TNF-α, as well as of VEGF. These results suggest that intraperitoneal VEGF synthesis is induced in response to inflammation, and that this may be an important component in the process leading to peritoneal transport alterations.

AB - Background: Peritoneal inflammation may induce changes in peritoneal microvessels, including neoangiogenesis/vasculogenesis, leading to increased peritoneal solute transport rate (PSTR) and loss of ultrafiltration capacity. We hypothesized that an inflammatory reaction in the peritoneal cavity during peritonitis induces increased synthesis of vascular endothelial growth factor (VEGF). We therefore studied the relationship between peritoneal inflammation markers, VEGF, and transport of fluid and solutes in rats during acute peritoneal inflammation induced by lipopolysaccharide (LPS) added to standard glucose-based dialysis solution. Methods: Under ether anesthesia, male Wistar rats were injected intraperitoneally with 30 mL Dianeal 3.86% without (Control; n=6) or with LPS (μg/mL): 0.001 (LPS 0.001; n=6), 0.01 (LPS 0.01; n=7), 0.1 (LPS 0.1; n=7), 1.0 (LPS 1.0; n=8). After 8 hours, dialysate volume (IPV), peritoneal solute transport rate (PSTR) and dialysate cell count (DCC) were measured and effluent samples were collected. Results: LPS i.p. resulted in increased PSTR and decreased IPV (p<0.005). DCC (cells/μL) and the neutrophil/macrophage ratio were higher for all LPS concentrations compared to the control group. After 8 hours, LPS-exposed rats had significantly higher dialysate levels of all investigated cytokines (TNF-α, MCP-1 and IL-10) than the control group. Addition of LPS resulted in increased dialysate VEGF concentrations (pg/mL) (LPS 0.001, 28.2±5.9; LPS 0.01, 38.9±11.6; LPS 0.1, 43.0±5.9; LPS 1.0, 46.6±11.3; Control, 14.5±9.8; p<0.0005 for all LPS vs. Control). Conclusions: The infusion of Dianeal 3.86% with different doses of LPS induced a strong acute intraperitoneal inflammatory reaction with increased DCC and cytokine levels, resulting in increased peritoneal solute transport and decreased IPV. LPS induced a dose-dependent parallel increase of the intraperitoneal concentrations of MCP-1, IL-10 and TNF-α, as well as of VEGF. These results suggest that intraperitoneal VEGF synthesis is induced in response to inflammation, and that this may be an important component in the process leading to peritoneal transport alterations.

KW - Animal model

KW - Cytokines

KW - Lipopolysaccharide

KW - Peritoneal dialysis

KW - Peritoneal inflammation

KW - Peritoneal transport

KW - Vascular endothelial growth factor

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