The differences in xenobiotic chemical content between organic and nonorganic products can be chemically measured. In this study, the deoxynucleotide composition of strawberry samples is used to demonstrate chromatographic methods of quantifying the differences between pesticide- and toxic-exposed strawberries. The samples were analyzed by 32P labeling and two-dimensional thin-layer chromatography. This technique is sensitive for detecting rare (1/1010) deoxynucleotide adducts and analogues (minor bases) in DNA. The results indicate differences in the amount and type of adduct formation between organic and nonorganic strawberry samples and within different parts of the same type of sample. The elucidation of the content and effects of pesticides and toxics in foods is critical in differentiating organic from common produce. It can also aid the agricultural industry in improving the application of chemicals in pest management. Furthermore, it helps to enhance the understanding of long-term epidemiology in nutrition research, especially in susceptible populations. These findings are of particular application in the pediatric population, where dietary habits are restricted to specific food groups.
- Thin- layered chromatography
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis