Variants of mouse myeloma cells that produce short immunoglobulin heavy chains

B. K. Birshtein, J. L. Preud'homme, Matthew D. Scharff

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

After mutagenesis of cultured mouse myeloma cells with ICR 191 or mephalan, variant clones were isolated that synthesized immunoglobulin heavy chains shorter than those produced by the parent. These variants fell into 2 phenotypes, based on the size, serology, and pattern of assembly of the heavy chain. Variant chains of both types no longer reacted with antisera directed either against the Fc (C terminal half of the heavy chains) or subclass specific IgG(2b) determinants. Comparative ion exchange chromatography of tryptic chymotryptic peptides confirmed that the variant heavy chains differed structurally from those of the parent and from each other. A conversion from one phenotype to the other was observed. (18 references.)

Original languageEnglish (US)
Pages (from-to)3478-3482
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number9
StatePublished - 1974

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Immunoglobulin Heavy Chains
Phenotype
Ion Exchange Chromatography
Serology
Mutagenesis
Immune Sera
Clone Cells
Immunoglobulin G
Peptides
acridine half-mustard

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Variants of mouse myeloma cells that produce short immunoglobulin heavy chains. / Birshtein, B. K.; Preud'homme, J. L.; Scharff, Matthew D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 71, No. 9, 1974, p. 3478-3482.

Research output: Contribution to journalArticle

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