Variants of human papillomaviruses 16 and 18 and their natural history in human immunodeficiency virus-positive women

Nicolas F. Schlecht, Robert D. Burk, Joel M. Palefsky, Howard Minkoff, Xiaonan (Nan) Xue, L. Stewart Massad, Melanie Bacon, Alexandra M. Levine, Kathryn Anastos, Stephen J. Gange, D. Heather Watts, Maria M. Da Costa, Zigui Chen, Ji Yon Bang, Melissa Fazzari, Charles B. Hall, Howard Strickler

Research output: Contribution to journalArticle

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Abstract

Highly oncogenic human papillomavirus (HPV) 16 and 18 variants might be expected to be particularly aggressive in HIV-positive women. The association of HPV16 and 18 variant lineages with race, human immunodeficiency virus (HIV) coinfection, CD4+ T-cell count, HIV-RNA level, time-to-clearance of HPV infection and presence of squamous intraepithelial lesions (SIL) among women in the Women's Interagency HIV Study was studied. Subjects were followed semi-annually with Pap smear and cervicovaginal lavage (CVL). HPV DNA was detected in CVLs using MY09/11 L1 PCR assay. Specimens positive for HPV16/18 underwent E6 PCR and sequencing to determine the variant present. Specimens from 195 HPV16- and 162 HPV18-positive women were classified into variant lineages based on sequencing results. African variants of HPV16 and HPV18 were significantly more prevalent among African-Americans than among Caucasians [42 versus 14 % (P=0.001) and 60 versus 13% (P<0.001), respectively]. However, it was not possible to detect associations between the HPV16 or 18 variant lineages and other factors studied. African variants of HPV16/18 were more common in women of African descent living outside Africa, which could reflect mixing behaviours and/or immunogenetic factors. However, in a large population of HIV-infected women, the variant of HPV16 or 18 was unrelated to persistence of infection or presence of SIL. If non-European variants are more oncogenic, the effect may involve a late stage in cervical tumorigenesis.

Original languageEnglish (US)
Pages (from-to)2709-2720
Number of pages12
JournalJournal of General Virology
Volume86
Issue number10
DOIs
StatePublished - Oct 2005

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Human papillomavirus 18
Human papillomavirus 16
Natural History
HIV
Papanicolaou Test
Immunogenetics
Polymerase Chain Reaction
Papillomavirus Infections
Therapeutic Irrigation
CD4 Lymphocyte Count
Coinfection
African Americans
Carcinogenesis
RNA
T-Lymphocytes
DNA
Infection
Population

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Variants of human papillomaviruses 16 and 18 and their natural history in human immunodeficiency virus-positive women. / Schlecht, Nicolas F.; Burk, Robert D.; Palefsky, Joel M.; Minkoff, Howard; Xue, Xiaonan (Nan); Massad, L. Stewart; Bacon, Melanie; Levine, Alexandra M.; Anastos, Kathryn; Gange, Stephen J.; Watts, D. Heather; Da Costa, Maria M.; Chen, Zigui; Bang, Ji Yon; Fazzari, Melissa; Hall, Charles B.; Strickler, Howard.

In: Journal of General Virology, Vol. 86, No. 10, 10.2005, p. 2709-2720.

Research output: Contribution to journalArticle

Schlecht, NF, Burk, RD, Palefsky, JM, Minkoff, H, Xue, XN, Massad, LS, Bacon, M, Levine, AM, Anastos, K, Gange, SJ, Watts, DH, Da Costa, MM, Chen, Z, Bang, JY, Fazzari, M, Hall, CB & Strickler, H 2005, 'Variants of human papillomaviruses 16 and 18 and their natural history in human immunodeficiency virus-positive women', Journal of General Virology, vol. 86, no. 10, pp. 2709-2720. https://doi.org/10.1099/vir.0.81060-0
Schlecht, Nicolas F. ; Burk, Robert D. ; Palefsky, Joel M. ; Minkoff, Howard ; Xue, Xiaonan (Nan) ; Massad, L. Stewart ; Bacon, Melanie ; Levine, Alexandra M. ; Anastos, Kathryn ; Gange, Stephen J. ; Watts, D. Heather ; Da Costa, Maria M. ; Chen, Zigui ; Bang, Ji Yon ; Fazzari, Melissa ; Hall, Charles B. ; Strickler, Howard. / Variants of human papillomaviruses 16 and 18 and their natural history in human immunodeficiency virus-positive women. In: Journal of General Virology. 2005 ; Vol. 86, No. 10. pp. 2709-2720.
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