Variable effects of short chain fatty acids and lactic acid in inducing intestinal mucosal injury in newborn rats

Jing Lin, Suhas M. Nafday, Sara N. Chauvin, Margret S. Magid, Sudha Pabbatireddy, Ian R. Holzman, Mark W. Babyatsky

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Short chain fatty acids and lactic acid are colonic bacterial fermentation products. Methods: To evaluate the effects of these organic acids on the intestinal mucosa, a total of 72 newborn Sprague-Dawley rats (10 days old) were studied. A 3.5F catheter was inserted per rectum 4.0 cm deep into the proximal colon for organic acid administration at a volume of 0.1 ml/10 g body weight. The pH of organic acid solutions and normal saline was adjusted to 4.0. Group 1 (n = 10) received normal saline as a control. Group 2 (n = 11) received 150 mM acetic acid. Group 3 (n = 11) received 300 mM acetic acid. Group 4 (n = 10) received 150 mM butyric acid. Group 5 (n = 11) received 300 mM butyric acid. Group 6 (n = 7) received 150 mM lactic acid, and group 7 (n = 12) received 300 mM lactic acid. Animals were killed 24 hours after colonic installation of test solutions. Results: Both 300 mM acetic acid and 300 mM butyric acid were associated with impaired weight gain, increased colon wet weight, and increased histologic injury scores in the colon and distal ileum (P < 0.05, analysis of variance). Both 150 mM acetic acid and butyric acid at 150 mmol/L induced minimal injury in the colon and distal ileum. Neither 150 mM nor 300 mM lactic acid induced any identifiable gross or microscopic intestinal mucosal injury. Conclusion: Luminal short chain fatty acids can induce dose-dependent intestinal mucosal injury in newborn rats, resembling the pathology seen in neonatal necrotizing enterocolitis. Overproduction/accumulation of short chain fatty acids, but not lactic acid, in the proximal colon and/or distal ileum may play a role in the pathogenesis of necrotizing enterocolitis in premature infants.

Original languageEnglish (US)
Pages (from-to)545-550
Number of pages6
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume35
Issue number4
DOIs
StatePublished - Oct 2 2002
Externally publishedYes

Fingerprint

Volatile Fatty Acids
short chain fatty acids
Butyric Acid
lactic acid
colon
butyric acid
Lactic Acid
Colon
neonates
Acetic Acid
acetic acid
Ileum
ileum
rats
Wounds and Injuries
organic acids and salts
enterocolitis
Necrotizing Enterocolitis
Acids
rectum

Keywords

  • Enterocolitis
  • Lactic acid
  • Necrotizing
  • Short Chain Fatty Acids

ASJC Scopus subject areas

  • Gastroenterology
  • Histology
  • Medicine (miscellaneous)
  • Food Science
  • Pediatrics, Perinatology, and Child Health

Cite this

Variable effects of short chain fatty acids and lactic acid in inducing intestinal mucosal injury in newborn rats. / Lin, Jing; Nafday, Suhas M.; Chauvin, Sara N.; Magid, Margret S.; Pabbatireddy, Sudha; Holzman, Ian R.; Babyatsky, Mark W.

In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 35, No. 4, 02.10.2002, p. 545-550.

Research output: Contribution to journalArticle

Lin, Jing ; Nafday, Suhas M. ; Chauvin, Sara N. ; Magid, Margret S. ; Pabbatireddy, Sudha ; Holzman, Ian R. ; Babyatsky, Mark W. / Variable effects of short chain fatty acids and lactic acid in inducing intestinal mucosal injury in newborn rats. In: Journal of Pediatric Gastroenterology and Nutrition. 2002 ; Vol. 35, No. 4. pp. 545-550.
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T1 - Variable effects of short chain fatty acids and lactic acid in inducing intestinal mucosal injury in newborn rats

AU - Lin, Jing

AU - Nafday, Suhas M.

AU - Chauvin, Sara N.

AU - Magid, Margret S.

AU - Pabbatireddy, Sudha

AU - Holzman, Ian R.

AU - Babyatsky, Mark W.

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AB - Background: Short chain fatty acids and lactic acid are colonic bacterial fermentation products. Methods: To evaluate the effects of these organic acids on the intestinal mucosa, a total of 72 newborn Sprague-Dawley rats (10 days old) were studied. A 3.5F catheter was inserted per rectum 4.0 cm deep into the proximal colon for organic acid administration at a volume of 0.1 ml/10 g body weight. The pH of organic acid solutions and normal saline was adjusted to 4.0. Group 1 (n = 10) received normal saline as a control. Group 2 (n = 11) received 150 mM acetic acid. Group 3 (n = 11) received 300 mM acetic acid. Group 4 (n = 10) received 150 mM butyric acid. Group 5 (n = 11) received 300 mM butyric acid. Group 6 (n = 7) received 150 mM lactic acid, and group 7 (n = 12) received 300 mM lactic acid. Animals were killed 24 hours after colonic installation of test solutions. Results: Both 300 mM acetic acid and 300 mM butyric acid were associated with impaired weight gain, increased colon wet weight, and increased histologic injury scores in the colon and distal ileum (P < 0.05, analysis of variance). Both 150 mM acetic acid and butyric acid at 150 mmol/L induced minimal injury in the colon and distal ileum. Neither 150 mM nor 300 mM lactic acid induced any identifiable gross or microscopic intestinal mucosal injury. Conclusion: Luminal short chain fatty acids can induce dose-dependent intestinal mucosal injury in newborn rats, resembling the pathology seen in neonatal necrotizing enterocolitis. Overproduction/accumulation of short chain fatty acids, but not lactic acid, in the proximal colon and/or distal ileum may play a role in the pathogenesis of necrotizing enterocolitis in premature infants.

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KW - Lactic acid

KW - Necrotizing

KW - Short Chain Fatty Acids

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