Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network

Francisco Lazaro-Dieguez, Cecilia Colonna, Miguel Cortegano, María Calvo, Susana E. Martínez, Gustavo Egea

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Efficient post-Golgi trafficking depends on microtubules, but actin filaments and actin-associated proteins are also postulated. Here we examined, by inverse fluorescence recovery after photobleaching, the role of actin dynamics in the exit from the TGN of fluorescent-tagged apical or basolateral and raft or non-raft-associated cargoes. Either the actin-stabilizing jasplakinolide or the actin-depolymerising latrunculin B variably but significantly inhibited post-Golgi traffic of non-raft associated apical p75NTR and basolateral VSV-G cargoes. The TGN-exit of the apical-destined VSV-G mutant was impaired only by latrunculin B. Strikingly, the raft-associated GPI-anchor protein was not affected by either actin toxin. Results indicate that actin dynamics participates in the TGN egress of both apical- and basolateral-targeted proteins but is not needed for apical raft-associated cargo.

Original languageEnglish (US)
Pages (from-to)3875-3881
Number of pages7
JournalFEBS Letters
Volume581
Issue number20
DOIs
StatePublished - Aug 7 2007
Externally publishedYes

Fingerprint

trans-Golgi Network
Actins
jasplakinolide
Fluorescence Recovery After Photobleaching
Proteins
Photobleaching
Actin Cytoskeleton
Microtubules
Anchors
Fluorescence
Recovery

Keywords

  • Actin
  • Cytoskeleton
  • Golgi apparatus
  • iFRAP
  • Polarized transport
  • Raft

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network. / Lazaro-Dieguez, Francisco; Colonna, Cecilia; Cortegano, Miguel; Calvo, María; Martínez, Susana E.; Egea, Gustavo.

In: FEBS Letters, Vol. 581, No. 20, 07.08.2007, p. 3875-3881.

Research output: Contribution to journalArticle

Lazaro-Dieguez, Francisco ; Colonna, Cecilia ; Cortegano, Miguel ; Calvo, María ; Martínez, Susana E. ; Egea, Gustavo. / Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network. In: FEBS Letters. 2007 ; Vol. 581, No. 20. pp. 3875-3881.
@article{5f472004664544e0b9bb18685c35a918,
title = "Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network",
abstract = "Efficient post-Golgi trafficking depends on microtubules, but actin filaments and actin-associated proteins are also postulated. Here we examined, by inverse fluorescence recovery after photobleaching, the role of actin dynamics in the exit from the TGN of fluorescent-tagged apical or basolateral and raft or non-raft-associated cargoes. Either the actin-stabilizing jasplakinolide or the actin-depolymerising latrunculin B variably but significantly inhibited post-Golgi traffic of non-raft associated apical p75NTR and basolateral VSV-G cargoes. The TGN-exit of the apical-destined VSV-G mutant was impaired only by latrunculin B. Strikingly, the raft-associated GPI-anchor protein was not affected by either actin toxin. Results indicate that actin dynamics participates in the TGN egress of both apical- and basolateral-targeted proteins but is not needed for apical raft-associated cargo.",
keywords = "Actin, Cytoskeleton, Golgi apparatus, iFRAP, Polarized transport, Raft",
author = "Francisco Lazaro-Dieguez and Cecilia Colonna and Miguel Cortegano and Mar{\'i}a Calvo and Mart{\'i}nez, {Susana E.} and Gustavo Egea",
year = "2007",
month = "8",
day = "7",
doi = "10.1016/j.febslet.2007.07.015",
language = "English (US)",
volume = "581",
pages = "3875--3881",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "20",

}

TY - JOUR

T1 - Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network

AU - Lazaro-Dieguez, Francisco

AU - Colonna, Cecilia

AU - Cortegano, Miguel

AU - Calvo, María

AU - Martínez, Susana E.

AU - Egea, Gustavo

PY - 2007/8/7

Y1 - 2007/8/7

N2 - Efficient post-Golgi trafficking depends on microtubules, but actin filaments and actin-associated proteins are also postulated. Here we examined, by inverse fluorescence recovery after photobleaching, the role of actin dynamics in the exit from the TGN of fluorescent-tagged apical or basolateral and raft or non-raft-associated cargoes. Either the actin-stabilizing jasplakinolide or the actin-depolymerising latrunculin B variably but significantly inhibited post-Golgi traffic of non-raft associated apical p75NTR and basolateral VSV-G cargoes. The TGN-exit of the apical-destined VSV-G mutant was impaired only by latrunculin B. Strikingly, the raft-associated GPI-anchor protein was not affected by either actin toxin. Results indicate that actin dynamics participates in the TGN egress of both apical- and basolateral-targeted proteins but is not needed for apical raft-associated cargo.

AB - Efficient post-Golgi trafficking depends on microtubules, but actin filaments and actin-associated proteins are also postulated. Here we examined, by inverse fluorescence recovery after photobleaching, the role of actin dynamics in the exit from the TGN of fluorescent-tagged apical or basolateral and raft or non-raft-associated cargoes. Either the actin-stabilizing jasplakinolide or the actin-depolymerising latrunculin B variably but significantly inhibited post-Golgi traffic of non-raft associated apical p75NTR and basolateral VSV-G cargoes. The TGN-exit of the apical-destined VSV-G mutant was impaired only by latrunculin B. Strikingly, the raft-associated GPI-anchor protein was not affected by either actin toxin. Results indicate that actin dynamics participates in the TGN egress of both apical- and basolateral-targeted proteins but is not needed for apical raft-associated cargo.

KW - Actin

KW - Cytoskeleton

KW - Golgi apparatus

KW - iFRAP

KW - Polarized transport

KW - Raft

UR - http://www.scopus.com/inward/record.url?scp=34547125988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547125988&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.07.015

DO - 10.1016/j.febslet.2007.07.015

M3 - Article

C2 - 17651738

AN - SCOPUS:34547125988

VL - 581

SP - 3875

EP - 3881

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 20

ER -