Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network

Francisco Lázaro-Diéguez, Cecilia Colonna, Miguel Cortegano, María Calvo, Susana E. Martínez, Gustavo Egea

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Efficient post-Golgi trafficking depends on microtubules, but actin filaments and actin-associated proteins are also postulated. Here we examined, by inverse fluorescence recovery after photobleaching, the role of actin dynamics in the exit from the TGN of fluorescent-tagged apical or basolateral and raft or non-raft-associated cargoes. Either the actin-stabilizing jasplakinolide or the actin-depolymerising latrunculin B variably but significantly inhibited post-Golgi traffic of non-raft associated apical p75NTR and basolateral VSV-G cargoes. The TGN-exit of the apical-destined VSV-G mutant was impaired only by latrunculin B. Strikingly, the raft-associated GPI-anchor protein was not affected by either actin toxin. Results indicate that actin dynamics participates in the TGN egress of both apical- and basolateral-targeted proteins but is not needed for apical raft-associated cargo.

Original languageEnglish (US)
Pages (from-to)3875-3881
Number of pages7
JournalFEBS Letters
Volume581
Issue number20
DOIs
StatePublished - Aug 7 2007
Externally publishedYes

Keywords

  • Actin
  • Cytoskeleton
  • Golgi apparatus
  • Polarized transport
  • Raft
  • iFRAP

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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