Variability of serum levels of tumor necrosis factor-alpha, interleukin 6, and soluble interleukin 6 receptor over 2 years in young women

Gloria Y F Ho, Xiaonan (Nan) Xue, Robert D. Burk, Robert C. Kaplan, Elaine Cornell, Mary Cushman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and soluble interleukin 6 receptor (sIL-6R) have been studied as risk factors of cardiovascular disease in longitudinal studies. However, it is unknown about their long-term intra-individual variations and whether single measurements of these cytokines and receptor are reliable biomarkers in epidemiological studies. In this study, serum levels of TNF-α, IL-6, and sIL-6R were assayed by ELISAs in 36 young, healthy women from whom three blood samples were collected at 12-month intervals over 2 years, and the intraclass correlation coefficients (ICC) were estimated. The ICC of 0.73 (95% CI = 0.49-0.79) for TNF-α was comparable to those of other commonly used biomarkers, justifying its use in epidemiological studies. The ICC of 0.48 (95% CI = 0.25-0.58) for IL-6 was not optimal. However, IL-6 has been demonstrated as a consistent risk factor for cardiovascular disease, suggesting it could still be a useful biomarker if its disease association is substantial. The ICC of 0.36 (95% CI = 0.10-0.47) for sIL-6R was relatively low, and multiple samples would need to be collected in prospective studies for this receptor.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalCytokine
Volume30
Issue number1
DOIs
StatePublished - Apr 7 2005

Fingerprint

Interleukin-6 Receptors
Interleukin-6
Biomarkers
Tumor Necrosis Factor-alpha
Serum
Epidemiologic Studies
Cardiovascular Diseases
Cytokine Receptors
Longitudinal Studies
Blood
Enzyme-Linked Immunosorbent Assay
Association reactions
Prospective Studies

Keywords

  • Interleukin 6
  • Soluble interleukin 6 receptor
  • Tumor necrosis factor-alpha
  • Variability

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Variability of serum levels of tumor necrosis factor-alpha, interleukin 6, and soluble interleukin 6 receptor over 2 years in young women. / Ho, Gloria Y F; Xue, Xiaonan (Nan); Burk, Robert D.; Kaplan, Robert C.; Cornell, Elaine; Cushman, Mary.

In: Cytokine, Vol. 30, No. 1, 07.04.2005, p. 1-6.

Research output: Contribution to journalArticle

@article{85696a5f411946b19d560abb5fc047d2,
title = "Variability of serum levels of tumor necrosis factor-alpha, interleukin 6, and soluble interleukin 6 receptor over 2 years in young women",
abstract = "Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and soluble interleukin 6 receptor (sIL-6R) have been studied as risk factors of cardiovascular disease in longitudinal studies. However, it is unknown about their long-term intra-individual variations and whether single measurements of these cytokines and receptor are reliable biomarkers in epidemiological studies. In this study, serum levels of TNF-α, IL-6, and sIL-6R were assayed by ELISAs in 36 young, healthy women from whom three blood samples were collected at 12-month intervals over 2 years, and the intraclass correlation coefficients (ICC) were estimated. The ICC of 0.73 (95{\%} CI = 0.49-0.79) for TNF-α was comparable to those of other commonly used biomarkers, justifying its use in epidemiological studies. The ICC of 0.48 (95{\%} CI = 0.25-0.58) for IL-6 was not optimal. However, IL-6 has been demonstrated as a consistent risk factor for cardiovascular disease, suggesting it could still be a useful biomarker if its disease association is substantial. The ICC of 0.36 (95{\%} CI = 0.10-0.47) for sIL-6R was relatively low, and multiple samples would need to be collected in prospective studies for this receptor.",
keywords = "Interleukin 6, Soluble interleukin 6 receptor, Tumor necrosis factor-alpha, Variability",
author = "Ho, {Gloria Y F} and Xue, {Xiaonan (Nan)} and Burk, {Robert D.} and Kaplan, {Robert C.} and Elaine Cornell and Mary Cushman",
year = "2005",
month = "4",
day = "7",
doi = "10.1016/j.cyto.2004.08.008",
language = "English (US)",
volume = "30",
pages = "1--6",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Variability of serum levels of tumor necrosis factor-alpha, interleukin 6, and soluble interleukin 6 receptor over 2 years in young women

AU - Ho, Gloria Y F

AU - Xue, Xiaonan (Nan)

AU - Burk, Robert D.

AU - Kaplan, Robert C.

AU - Cornell, Elaine

AU - Cushman, Mary

PY - 2005/4/7

Y1 - 2005/4/7

N2 - Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and soluble interleukin 6 receptor (sIL-6R) have been studied as risk factors of cardiovascular disease in longitudinal studies. However, it is unknown about their long-term intra-individual variations and whether single measurements of these cytokines and receptor are reliable biomarkers in epidemiological studies. In this study, serum levels of TNF-α, IL-6, and sIL-6R were assayed by ELISAs in 36 young, healthy women from whom three blood samples were collected at 12-month intervals over 2 years, and the intraclass correlation coefficients (ICC) were estimated. The ICC of 0.73 (95% CI = 0.49-0.79) for TNF-α was comparable to those of other commonly used biomarkers, justifying its use in epidemiological studies. The ICC of 0.48 (95% CI = 0.25-0.58) for IL-6 was not optimal. However, IL-6 has been demonstrated as a consistent risk factor for cardiovascular disease, suggesting it could still be a useful biomarker if its disease association is substantial. The ICC of 0.36 (95% CI = 0.10-0.47) for sIL-6R was relatively low, and multiple samples would need to be collected in prospective studies for this receptor.

AB - Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and soluble interleukin 6 receptor (sIL-6R) have been studied as risk factors of cardiovascular disease in longitudinal studies. However, it is unknown about their long-term intra-individual variations and whether single measurements of these cytokines and receptor are reliable biomarkers in epidemiological studies. In this study, serum levels of TNF-α, IL-6, and sIL-6R were assayed by ELISAs in 36 young, healthy women from whom three blood samples were collected at 12-month intervals over 2 years, and the intraclass correlation coefficients (ICC) were estimated. The ICC of 0.73 (95% CI = 0.49-0.79) for TNF-α was comparable to those of other commonly used biomarkers, justifying its use in epidemiological studies. The ICC of 0.48 (95% CI = 0.25-0.58) for IL-6 was not optimal. However, IL-6 has been demonstrated as a consistent risk factor for cardiovascular disease, suggesting it could still be a useful biomarker if its disease association is substantial. The ICC of 0.36 (95% CI = 0.10-0.47) for sIL-6R was relatively low, and multiple samples would need to be collected in prospective studies for this receptor.

KW - Interleukin 6

KW - Soluble interleukin 6 receptor

KW - Tumor necrosis factor-alpha

KW - Variability

UR - http://www.scopus.com/inward/record.url?scp=15244347218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15244347218&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2004.08.008

DO - 10.1016/j.cyto.2004.08.008

M3 - Article

VL - 30

SP - 1

EP - 6

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 1

ER -