Variability in expression of insecticidal Cry1Ab gene in Indica Basmati rice

Tayyab Husnain, Jan Asad, Shahina B. Maqbool, Swapan K. Datta, S. Riazuddin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The expression of an insecticidal gene cry1Ab, under three different promoters was studied in leaves, stem and panicles to determine organ-specificity in Basmati rice. Enhanced resistance against two Lepidopteran insects, stem bore (Scripophaga incertulas) and leaf folder (Cnaphalocrocis medinalis) was observed. The result of western hybridization and insect bioassays demonstrated that all these promoters express the cry1Ab gene at similar levels in leaves and panicles. The cry1Ab gene was expressed in stems at 0.05% of the total protein under the control of the PEPC promoter alone or in combination with the pollen-specific promoter. On the other hand it was expressed at 0.15% under the control of the ubiquitin promoter. Southern blot hybridization of these plants indicated integration of the complete plant transcriptional unit at multiple insertion sites. These results demonstrated that a specific promoter could be used to limit the expression of cry1Ab gene in the desired parts of Basmati rice plants.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalEuphytica
Volume128
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

promoter regions
rice
Insects
Genes
Gene Expression
Organ Specificity
genes
Ubiquitin
Southern Blotting
Pollen
Biological Assay
stems
hybridization
inflorescences
Cnaphalocrocis medinalis
leafrollers
insects
ubiquitin
Southern blotting
Oryza

Keywords

  • Bacillus thuringiensis
  • Basmati rice
  • cry1Ab gene
  • Lepidopteran insects
  • Tissue-specific promoters
  • Transgenic rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cite this

Variability in expression of insecticidal Cry1Ab gene in Indica Basmati rice. / Husnain, Tayyab; Asad, Jan; Maqbool, Shahina B.; Datta, Swapan K.; Riazuddin, S.

In: Euphytica, Vol. 128, No. 1, 2002, p. 121-128.

Research output: Contribution to journalArticle

Husnain, Tayyab ; Asad, Jan ; Maqbool, Shahina B. ; Datta, Swapan K. ; Riazuddin, S. / Variability in expression of insecticidal Cry1Ab gene in Indica Basmati rice. In: Euphytica. 2002 ; Vol. 128, No. 1. pp. 121-128.
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