Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development

Javier Calvo-Garrido, Sergio Carilla-Latorre, Francisco Lazaro-Dieguez, Gustavo Egea, Ricardo Escalante

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Vacuole membrane protein 1 (Vmp1) is membrane protein of unknown molecular function that has been associated with pancreatitis and cancer. The social amoeba Dictyostelium discoideum has a vmp1-related gene that we identified previously in a functional genomic study. Loss-of-function of this gene leads to a severe phenotype that compromises Dictyostelium growth and development. The expression of mammalian Vmp1 in a vmp1- Dictyostelium mutant complemented the phenotype, suggesting a functional conservation of the protein among evolutionarily distant species and highlights Dictyostelium as a valid experimental system to address the function of this gene. Dictyostelium Vmp1 is an endoplasmic reticulum protein necessary for the integrity of this organelle. Cells deficient in Vmp1 display pleiotropic defects in the secretory pathway and organelle biogenesis. The contractile vacuole, which is necessary to survive under hypoosmotic conditions, is not functional in the mutant. The structure of the Golgi apparatus, the function of the endocytic pathway and conventional protein secretion are also affected in these cells. Transmission electron microscopy of vmp1- cells showed the accumulation of autophagic features that suggests a role of Vmp1 in macroautophagy. In addition to these defects observed at the vegetative stage, the onset of multicellular development and early developmental gene expression are also compromised.

Original languageEnglish (US)
Pages (from-to)3442-3453
Number of pages12
JournalMolecular Biology of the Cell
Volume19
Issue number8
DOIs
StatePublished - Aug 2008
Externally publishedYes

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Organelle Biogenesis
Vacuoles
Dictyostelium
Endoplasmic Reticulum
Membrane Proteins
Proteins
Genes
Developmental Genes
Phenotype
Amoeba
Secretory Pathway
Autophagy
Golgi Apparatus
Transmission Electron Microscopy
Growth and Development
Pancreatitis
Organelles
Gene Expression
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development. / Calvo-Garrido, Javier; Carilla-Latorre, Sergio; Lazaro-Dieguez, Francisco; Egea, Gustavo; Escalante, Ricardo.

In: Molecular Biology of the Cell, Vol. 19, No. 8, 08.2008, p. 3442-3453.

Research output: Contribution to journalArticle

Calvo-Garrido, Javier ; Carilla-Latorre, Sergio ; Lazaro-Dieguez, Francisco ; Egea, Gustavo ; Escalante, Ricardo. / Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 8. pp. 3442-3453.
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