Vβ2 natural killer T cell antigen receptor-mediated recognition of CD1d-glycolipid antigen

Onisha Patel, Daniel G. Pellicci, Adam P. Uldrich, Lucy C. Sullivan, Mugdha Bhati, Melissa McKnight, Stewart K. Richardson, Amy R. Howell, Thierry Mallevaey, Jingjing Zhang, Romain Bedel, Gurdyal S. Besra, Andrew G. Brooks, Lars Kjer-Nielsen, James McCluskey, Steven A. Porcelli, Laurent Gapin, Jamie Rossjohn, Dale I. Godfrey

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Natural killer T cell antigen receptors (NKT TCRs) recognize lipidbased antigens (Ags) presented by CD1d. Although the TCR α-chain is invariant, NKT TCR Vβ exhibits greater diversity, with one (Vβ11) and three (Vβ8, Vβ7, and Vβ2) Vβ chains in humans and mice, respectively. With the exception of the Vβ2 NKT TCR, NKT TCRs possess canonical tyrosine residues within complementarity determining region (CDR) 2β that are critical for CD1d binding. Thus, how Vβ2 NKT TCR docks with CD1d-Ag was unclear. Despite the absence of the CDR2β-encoded tyrosine residues, we show that the Vβ2 NKT TCR engaged CD1d-Ag in a similar manner and with a comparable affinity and energetic footprint to the manner observed for the Vβ8.2 and Vβ7 NKT TCRs. Accordingly, the germline-encoded regions of the TCR β-chain do not exclusively dictate the innate NKT TCR-CD1d-Ag docking mode. Nevertheless, clear fine specificity differences for the CD1d-Ag existed between the Vβ2 NKT TCR and the Vβ8.2 and Vβ7 NKT TCRs, with the Vβ2 NKT TCR exhibiting greater sensitivity to modifications to the glycolipid Ag. Furthermore, within the Vβ2 NKT TCR-CD1d-αGalCer complex, the CDR2β loop mediated fewer contacts with CD1d, whereas the CDR1β and CDR3β loops contacted CD1d to a much greater extent compared with most Vβ11, Vβ8.2, and Vβ7 NKT TCRs. Accordingly, there is a greater interplay between the germline- and nongermline-encoded loops within the TCR β-chain of the Vβ2 NKT TCR that enables CD1d-Ag ligation.

Original languageEnglish (US)
Pages (from-to)19007-19012
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number47
DOIs
StatePublished - Nov 22 2011

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CD1d Antigen
Natural Killer T-Cells
Glycolipids
T-Cell Antigen Receptor
Tyrosine
Complementarity Determining Regions
Ligation

Keywords

  • Conserved docking
  • T cell repertoire

ASJC Scopus subject areas

  • General

Cite this

Vβ2 natural killer T cell antigen receptor-mediated recognition of CD1d-glycolipid antigen. / Patel, Onisha; Pellicci, Daniel G.; Uldrich, Adam P.; Sullivan, Lucy C.; Bhati, Mugdha; McKnight, Melissa; Richardson, Stewart K.; Howell, Amy R.; Mallevaey, Thierry; Zhang, Jingjing; Bedel, Romain; Besra, Gurdyal S.; Brooks, Andrew G.; Kjer-Nielsen, Lars; McCluskey, James; Porcelli, Steven A.; Gapin, Laurent; Rossjohn, Jamie; Godfrey, Dale I.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 47, 22.11.2011, p. 19007-19012.

Research output: Contribution to journalArticle

Patel, O, Pellicci, DG, Uldrich, AP, Sullivan, LC, Bhati, M, McKnight, M, Richardson, SK, Howell, AR, Mallevaey, T, Zhang, J, Bedel, R, Besra, GS, Brooks, AG, Kjer-Nielsen, L, McCluskey, J, Porcelli, SA, Gapin, L, Rossjohn, J & Godfrey, DI 2011, 'Vβ2 natural killer T cell antigen receptor-mediated recognition of CD1d-glycolipid antigen', Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 47, pp. 19007-19012. https://doi.org/10.1073/pnas.1109066108
Patel, Onisha ; Pellicci, Daniel G. ; Uldrich, Adam P. ; Sullivan, Lucy C. ; Bhati, Mugdha ; McKnight, Melissa ; Richardson, Stewart K. ; Howell, Amy R. ; Mallevaey, Thierry ; Zhang, Jingjing ; Bedel, Romain ; Besra, Gurdyal S. ; Brooks, Andrew G. ; Kjer-Nielsen, Lars ; McCluskey, James ; Porcelli, Steven A. ; Gapin, Laurent ; Rossjohn, Jamie ; Godfrey, Dale I. / Vβ2 natural killer T cell antigen receptor-mediated recognition of CD1d-glycolipid antigen. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 47. pp. 19007-19012.
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AU - Bhati, Mugdha

AU - McKnight, Melissa

AU - Richardson, Stewart K.

AU - Howell, Amy R.

AU - Mallevaey, Thierry

AU - Zhang, Jingjing

AU - Bedel, Romain

AU - Besra, Gurdyal S.

AU - Brooks, Andrew G.

AU - Kjer-Nielsen, Lars

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