Utility of whole-genome next-generation sequencing of plasma in identifying opportunistic infections in HIV/AIDS

Yang Zhou, Vagish S. Hemmige, Sudeb C. Dalai, David K. Hong, Kenneth Muldrew, Mayar Al Mohajer

Research output: Contribution to journalArticle

Abstract

Background: AIDS-associated Opportunistic Infections (OIs) have significant morbidity and mortality and can be diagnostically challenging, requiring invasive procedures as well as a combination of culture and targeted molecular approaches. Objective: We aimed to demonstrate the clinical utility of Next-generation Sequencing (NGS) in pathogen identification; NGS is a maturing technology enabling the detection of miniscule amounts of cell-free microbial DNA from the bloodstream. Methods: We utilized a novel Next-generation Sequencing (NGS) test on plasma samples to diagnose a series of HIV-associated OIs that were diagnostically confirmed through conventional microbial testing. Results: In all cases, NGS test results were available sooner than conventional testing. This is the first case series demonstrating the utility of whole-genome NGS testing to identify OIs from plasma in HIV/AIDS patients. Conclusion: NGS approaches present a clinically-actionable, comprehensive means of diagnosing OIs and other systemic infections while avoiding the labor, expense, and delays of multiple tests and invasive procedures.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalOpen AIDS Journal
Volume13
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Opportunistic Infections
Acquired Immunodeficiency Syndrome
HIV
Genome
Technology
Morbidity
Mortality
DNA
Infection

Keywords

  • Acquired immune deficiency syndrome
  • Cellfree dna
  • Cryptosporidium
  • Human immunodeficiency virus
  • Mycobacterium avium complex
  • Mycobacterium tuberculosis
  • Next-generation sequencing
  • Opportunistic infections
  • Toxoplasma gondii
  • Toxoplasmosis

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases
  • Virology

Cite this

Utility of whole-genome next-generation sequencing of plasma in identifying opportunistic infections in HIV/AIDS. / Zhou, Yang; Hemmige, Vagish S.; Dalai, Sudeb C.; Hong, David K.; Muldrew, Kenneth; Al Mohajer, Mayar.

In: Open AIDS Journal, Vol. 13, No. 1, 01.01.2019, p. 7-11.

Research output: Contribution to journalArticle

Zhou, Yang ; Hemmige, Vagish S. ; Dalai, Sudeb C. ; Hong, David K. ; Muldrew, Kenneth ; Al Mohajer, Mayar. / Utility of whole-genome next-generation sequencing of plasma in identifying opportunistic infections in HIV/AIDS. In: Open AIDS Journal. 2019 ; Vol. 13, No. 1. pp. 7-11.
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