Utility of whole-genome next-generation sequencing of plasma in identifying opportunistic infections in HIV/AIDS

Yang Zhou, Vagish Hemmige, Sudeb C. Dalai, David K. Hong, Kenneth Muldrew, Mayar Al Mohajer

Research output: Contribution to journalArticle

Abstract

Background: AIDS-associated Opportunistic Infections (OIs) have significant morbidity and mortality and can be diagnostically challenging, requiring invasive procedures as well as a combination of culture and targeted molecular approaches. Objective: We aimed to demonstrate the clinical utility of Next-generation Sequencing (NGS) in pathogen identification; NGS is a maturing technology enabling the detection of miniscule amounts of cell-free microbial DNA from the bloodstream. Methods: We utilized a novel Next-generation Sequencing (NGS) test on plasma samples to diagnose a series of HIV-associated OIs that were diagnostically confirmed through conventional microbial testing. Results: In all cases, NGS test results were available sooner than conventional testing. This is the first case series demonstrating the utility of whole-genome NGS testing to identify OIs from plasma in HIV/AIDS patients. Conclusion: NGS approaches present a clinically-actionable, comprehensive means of diagnosing OIs and other systemic infections while avoiding the labor, expense, and delays of multiple tests and invasive procedures.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalOpen AIDS Journal
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

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Keywords

  • Acquired immune deficiency syndrome
  • Cellfree dna
  • Cryptosporidium
  • Human immunodeficiency virus
  • Mycobacterium avium complex
  • Mycobacterium tuberculosis
  • Next-generation sequencing
  • Opportunistic infections
  • Toxoplasma gondii
  • Toxoplasmosis

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases
  • Virology

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