Utility of a C‐Jun Microsatellite Marker in Determining Gene Dosage for fatty (fa)

Streamson C. Chua, Moritz H. Hansen, Gary E. Truet, Rudolph L. Leibel

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The Zucker fatty (fa) mutation provides a genetic model for obesity and non‐insulin dependent diabetes mellitus. The molecular pathogenesis of the metabolic phenotype of these animals is not known. Detailed molecular maps of the region surrounding the fa locus on rat chromosome 5 can be used for positional cloning experiments as well as to permit genotyping of animals from appropriate crosses before the confounding metabolic effects of obesity have occurred. We describe the development of a polymerase chain reaction (PCR) assay for a polymorphic simple sequence repeat (SSR) in the promoter region of the protooncogene c‐Jun. This assay was used to position cJun 4.5cM proximal to the fa locus in 111 F2 progeny of a 13MBN fa/+ F1 intercross. Concurrent use of the cJun SSR with a previously described assay for a microsatellite in the glucose transporter, Glutl, permits rapid and accurate assessment of genotypes at the fa locus in animals of any age using minimal amounts of DNA. A strategy is described which minimizes the error rate in assigning genotype at the fatty locus for backcross and intercross progeny. 1993 North American Association for the Study of Obesity (NAASO)

Original languageEnglish (US)
Pages (from-to)475-480
Number of pages6
JournalObesity research
Volume1
Issue number6
DOIs
Publication statusPublished - Nov 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Public Health, Environmental and Occupational Health

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