Utility of a C-jun microsatellite marker in determining gene dosage for fatty (fa).

Streamson C. Chua, Jr., M. H. Hansen, G. E. Truett, R. L. Leibel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The Zucker fatty (fa) mutation provides a genetic model for obesity and non-insulin dependent diabetes mellitus. The molecular pathogenesis of the metabolic phenotype of these animals is not known. Detailed molecular maps of the region surrounding the fa locus on rat chromosome 5 can be used for positional cloning experiments as well as to permit genotyping of animals from appropriate crosses before the confounding metabolic effects of obesity have occurred. We describe the development of a polymerase chain reaction (PCR) assay for a polymorphic simple sequence repeat (SSR) in the promoter region of the protooncogene c-Jun. This assay was used to position c-Jun 4.5cM proximal to the fa locus in 111 F2 progeny of a 13MBN fa/+ F1 intercross. Concurrent use of the c-Jun SSR with a previously described assay for a microsatellite in the glucose transporter, Glut1, permits rapid and accurate assessment of genotypes at the fa locus in animals of any age using minimal amounts of DNA. A strategy is described which minimizes the error rate in assigning genotype at the fatty locus for backcross and intercross progeny.

Original languageEnglish (US)
Pages (from-to)475-480
Number of pages6
JournalObesity research.
Volume1
Issue number6
StatePublished - Nov 1993
Externally publishedYes

Fingerprint

gene dosage
Gene Dosage
Microsatellite Repeats
microsatellite repeats
loci
genetic markers
Obesity
Genotype
Chromosomes, Human, Pair 5
assays
obesity
Facilitative Glucose Transport Proteins
Genetic Models
proto-oncogenes
Genetic Promoter Regions
glucose transporters
Type 2 Diabetes Mellitus
Organism Cloning
genotype
animal age

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Utility of a C-jun microsatellite marker in determining gene dosage for fatty (fa). / Chua, Jr., Streamson C.; Hansen, M. H.; Truett, G. E.; Leibel, R. L.

In: Obesity research., Vol. 1, No. 6, 11.1993, p. 475-480.

Research output: Contribution to journalArticle

Chua, Jr., SC, Hansen, MH, Truett, GE & Leibel, RL 1993, 'Utility of a C-jun microsatellite marker in determining gene dosage for fatty (fa).', Obesity research., vol. 1, no. 6, pp. 475-480.
Chua, Jr., Streamson C. ; Hansen, M. H. ; Truett, G. E. ; Leibel, R. L. / Utility of a C-jun microsatellite marker in determining gene dosage for fatty (fa). In: Obesity research. 1993 ; Vol. 1, No. 6. pp. 475-480.
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