Abstract
In most structural biology NMR laboratories, instrument time is a limiting factor in the number of structural projects a laboratory is able to support. In the post-genomic era we can expect the number of structural targets to markedly increase. Here we address to what degree recently introduced cryoprobes, which are 3-4 times as sensitive as conventional probes, can alleviate this problem. To evaluate this approach, a set of triple-resonance experiments for protein assignments were acquired with a cryoprobe. We show that, with the cryoprobe, high quality triple-resonance data can be obtained within as 4 hours/experiment. These results show that a full set of data for protein assignments can now be practically collected in 1-2 days.
Original language | English (US) |
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Pages (from-to) | 161-167 |
Number of pages | 7 |
Journal | Spectroscopy |
Volume | 17 |
Issue number | 2-3 |
DOIs | |
State | Published - 2003 |
Keywords
- Cryoprobes
- High-throughput
- NMR spectroscopy
- Proteins
- Reduced phase cycle
- Triple-resonance
ASJC Scopus subject areas
- Spectroscopy