Using cryoprobes to decrease acquisition times of triple-resonance experiments used for protein resonance assignments

Michael J. Goger, James M. McDonnell, David Cowburn

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

In most structural biology NMR laboratories, instrument time is a limiting factor in the number of structural projects a laboratory is able to support. In the post-genomic era we can expect the number of structural targets to markedly increase. Here we address to what degree recently introduced cryoprobes, which are 3-4 times as sensitive as conventional probes, can alleviate this problem. To evaluate this approach, a set of triple-resonance experiments for protein assignments were acquired with a cryoprobe. We show that, with the cryoprobe, high quality triple-resonance data can be obtained within as 4 hours/experiment. These results show that a full set of data for protein assignments can now be practically collected in 1-2 days.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalSpectroscopy
Volume17
Issue number2-3
DOIs
Publication statusPublished - Jan 1 2003

    Fingerprint

Keywords

  • Cryoprobes
  • High-throughput
  • NMR spectroscopy
  • Proteins
  • Reduced phase cycle
  • Triple-resonance

ASJC Scopus subject areas

  • Spectroscopy

Cite this