Using antibody arrays to detect protein-protein interactions

Heather S. Duffy, Ionela Iacobas, David C. Spray, Anthony W. Ashton

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The antibody array technique is a step in the direction of high-throughput screens, similar to the cDNA or oligonucleotide arrays that have been developed for gene profiling. Antibody arrays allow for rapid screening of multiple potential interactions, confirmation of which is relatively simple, given the state of technology now available for co-immunoprecipitation, confocal immunolocalization and mass spectrometry. In this Chapter we review this powerful method, using as an example the study of protein-protein interactions involving connexin, the protein subunit that forms gap junction channels.

Original languageEnglish (US)
Title of host publicationPractical Methods in Cardiovascular Research
PublisherSpringer Berlin Heidelberg
Pages916-935
Number of pages20
ISBN (Print)3540407634, 9783540407638
DOIs
StatePublished - 2005

Fingerprint

Oligonucleotide Array Sequence Analysis
Connexins
Antibodies
Gap Junctions
Protein Subunits
Immunoprecipitation
Mass Spectrometry
Proteins
Technology
Genes
Direction compound

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Duffy, H. S., Iacobas, I., Spray, D. C., & Ashton, A. W. (2005). Using antibody arrays to detect protein-protein interactions. In Practical Methods in Cardiovascular Research (pp. 916-935). Springer Berlin Heidelberg. https://doi.org/10.1007/3-540-26574-0_46

Using antibody arrays to detect protein-protein interactions. / Duffy, Heather S.; Iacobas, Ionela; Spray, David C.; Ashton, Anthony W.

Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg, 2005. p. 916-935.

Research output: Chapter in Book/Report/Conference proceedingChapter

Duffy, HS, Iacobas, I, Spray, DC & Ashton, AW 2005, Using antibody arrays to detect protein-protein interactions. in Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg, pp. 916-935. https://doi.org/10.1007/3-540-26574-0_46
Duffy HS, Iacobas I, Spray DC, Ashton AW. Using antibody arrays to detect protein-protein interactions. In Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg. 2005. p. 916-935 https://doi.org/10.1007/3-540-26574-0_46
Duffy, Heather S. ; Iacobas, Ionela ; Spray, David C. ; Ashton, Anthony W. / Using antibody arrays to detect protein-protein interactions. Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg, 2005. pp. 916-935
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