Using antibody arrays to detect protein-protein interactions

Heather S. Duffy, Ionela Iacobas, David C. Spray, Anthony W. Ashton

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The antibody array technique is a step in the direction of high-throughput screens, similar to the cDNA or oligonucleotide arrays that have been developed for gene profiling. Antibody arrays allow for rapid screening of multiple potential interactions, confirmation of which is relatively simple, given the state of technology now available for co-immunoprecipitation, confocal immunolocalization and mass spectrometry. In this Chapter we review this powerful method, using as an example the study of protein-protein interactions involving connexin, the protein subunit that forms gap junction channels.

Original languageEnglish (US)
Title of host publicationPractical Methods in Cardiovascular Research
PublisherSpringer Berlin Heidelberg
Pages916-935
Number of pages20
ISBN (Print)3540407634, 9783540407638
DOIs
Publication statusPublished - Dec 1 2005

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Duffy, H. S., Iacobas, I., Spray, D. C., & Ashton, A. W. (2005). Using antibody arrays to detect protein-protein interactions. In Practical Methods in Cardiovascular Research (pp. 916-935). Springer Berlin Heidelberg. https://doi.org/10.1007/3-540-26574-0_46