Use of Topical Human Amniotic Fluid in the Treatment of Acute Ocular Alkali Injuries in Mice

Samantha Herretes, Olan Suwan-Apichon, Ashkan Pirouzmanesh, Johann M.G. Reyes, Aimee T. Broman, Marisol Cano, Peter L. Gehlbach, Edith D. Gurewitsch Allen, Elia J. Duh, Ashley Behrens

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Abstract

Purpose: To evaluate the efficacy of topical human amniotic fluid (HAF) in the treatment of ocular acute alkali burns in mice. Design: Experimental study. Methods: A chemical burn with 2 μl of sodium hydroxide 0.15 mol/l was created in one eye of 30 mice. The animals were divided into gender- and age-matched groups according to the topical treatment that was administered: group 1 was treated with preterm HAF (n = 10 mice); group 2 was treated with term HAF (n = 10 mice), and group 3 was treated with saline solution (n = 10 mice). Treatment consisted of one drop that was applied to the burned eye five times per day (week one), and three times per day (week two). The epithelial defect was photographed and measured on days two and four. Ocular burn damage was assessed at days two, seven, and 14 after a pre-established classification. On day 14, both eyes of each mouse were enucleated and assessed histopathologically. Results: Median epithelial defect (interquartile range [IQR], 25th, 75th percentile) at day four was 9.93% (IQR, 8.57, 11.27) for group 1, 7.30% (IQR, 5.96, 8.97) for group 2, and 18.92% (IQR, 11.71, 27.64) for group 3 (P < .0076). The overall change (difference in slope) in ocular burn score between days 2 and 14 was -0.127 (P = .009) in group 1 vs 3, -0.134 (P = .012) in group 2 vs 3, and 0.007 (P = .88) in group 1 vs 2. On histologic examination saline solution-treated corneas had more inflammatory cells and blood vessels than HAF-treated corneas. Conclusion: Topical preterm/term HAF was an effective topical therapy for limiting the damage after acute alkali burns of the eye in this animal model.

Original languageEnglish (US)
JournalAmerican Journal of Ophthalmology
Volume142
Issue number2
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

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Eye Injuries
Alkalies
Amniotic Fluid
Eye Burns
Sodium Chloride
Cornea
Research Design
Therapeutics
Chemical Burns
Sodium Hydroxide
Burns
Blood Vessels
Animal Models
Age Groups

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Herretes, S., Suwan-Apichon, O., Pirouzmanesh, A., Reyes, J. M. G., Broman, A. T., Cano, M., ... Behrens, A. (2006). Use of Topical Human Amniotic Fluid in the Treatment of Acute Ocular Alkali Injuries in Mice. American Journal of Ophthalmology, 142(2). https://doi.org/10.1016/j.ajo.2006.03.025

Use of Topical Human Amniotic Fluid in the Treatment of Acute Ocular Alkali Injuries in Mice. / Herretes, Samantha; Suwan-Apichon, Olan; Pirouzmanesh, Ashkan; Reyes, Johann M.G.; Broman, Aimee T.; Cano, Marisol; Gehlbach, Peter L.; Gurewitsch Allen, Edith D.; Duh, Elia J.; Behrens, Ashley.

In: American Journal of Ophthalmology, Vol. 142, No. 2, 01.08.2006.

Research output: Contribution to journalArticle

Herretes, S, Suwan-Apichon, O, Pirouzmanesh, A, Reyes, JMG, Broman, AT, Cano, M, Gehlbach, PL, Gurewitsch Allen, ED, Duh, EJ & Behrens, A 2006, 'Use of Topical Human Amniotic Fluid in the Treatment of Acute Ocular Alkali Injuries in Mice', American Journal of Ophthalmology, vol. 142, no. 2. https://doi.org/10.1016/j.ajo.2006.03.025
Herretes, Samantha ; Suwan-Apichon, Olan ; Pirouzmanesh, Ashkan ; Reyes, Johann M.G. ; Broman, Aimee T. ; Cano, Marisol ; Gehlbach, Peter L. ; Gurewitsch Allen, Edith D. ; Duh, Elia J. ; Behrens, Ashley. / Use of Topical Human Amniotic Fluid in the Treatment of Acute Ocular Alkali Injuries in Mice. In: American Journal of Ophthalmology. 2006 ; Vol. 142, No. 2.
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abstract = "Purpose: To evaluate the efficacy of topical human amniotic fluid (HAF) in the treatment of ocular acute alkali burns in mice. Design: Experimental study. Methods: A chemical burn with 2 μl of sodium hydroxide 0.15 mol/l was created in one eye of 30 mice. The animals were divided into gender- and age-matched groups according to the topical treatment that was administered: group 1 was treated with preterm HAF (n = 10 mice); group 2 was treated with term HAF (n = 10 mice), and group 3 was treated with saline solution (n = 10 mice). Treatment consisted of one drop that was applied to the burned eye five times per day (week one), and three times per day (week two). The epithelial defect was photographed and measured on days two and four. Ocular burn damage was assessed at days two, seven, and 14 after a pre-established classification. On day 14, both eyes of each mouse were enucleated and assessed histopathologically. Results: Median epithelial defect (interquartile range [IQR], 25th, 75th percentile) at day four was 9.93{\%} (IQR, 8.57, 11.27) for group 1, 7.30{\%} (IQR, 5.96, 8.97) for group 2, and 18.92{\%} (IQR, 11.71, 27.64) for group 3 (P < .0076). The overall change (difference in slope) in ocular burn score between days 2 and 14 was -0.127 (P = .009) in group 1 vs 3, -0.134 (P = .012) in group 2 vs 3, and 0.007 (P = .88) in group 1 vs 2. On histologic examination saline solution-treated corneas had more inflammatory cells and blood vessels than HAF-treated corneas. Conclusion: Topical preterm/term HAF was an effective topical therapy for limiting the damage after acute alkali burns of the eye in this animal model.",
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AU - Reyes, Johann M.G.

AU - Broman, Aimee T.

AU - Cano, Marisol

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