Use of streptokinase as a recall antigen for lymphocyte proliferation testing in vitro

Lisa H. Youkeles, Mohammed Y. Soliman, David L. Rosenstreich

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The use of streptokinase (SK) as a recall antigen (Ag) for stimulation of human lymphocyte proliferation in vitro was evaluated. SK is a potent stimulant, inducing a greater response than three other Ags, tetanus toxoid, Candida albicans extract, or streptolysin. The optimum stimulatory concentration is 1000 U/ml, and peak stimulation occurs between days 6 and 8. Human cord-blood lymphocytes do not respond to SK, suggesting that it is an Ag and not a mitogen. The response rate in an adult population was 82%, higher than the rate of two other Ags tested (tetanus toxoid or Candida). These findings demonstrate the ability of SK to act as a potent recall Ag for human lymphocyte proliferation in vitro.

Original languageEnglish (US)
Pages (from-to)166-171
Number of pages6
JournalThe Journal of Allergy and Clinical Immunology
Volume88
Issue number2
DOIs
StatePublished - 1991

Fingerprint

Streptokinase
Lymphocytes
Antigens
Tetanus Toxoid
Streptolysins
Lymphocyte Activation
Candida albicans
Fetal Blood
Mitogens
Candida
In Vitro Techniques
Population

Keywords

  • antigens
  • cell-mediated immunity
  • cord blood
  • lymphocyte proliferation
  • lymphocytes
  • mitogens
  • Streptokinase
  • tetanus toxoid

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Use of streptokinase as a recall antigen for lymphocyte proliferation testing in vitro. / Youkeles, Lisa H.; Soliman, Mohammed Y.; Rosenstreich, David L.

In: The Journal of Allergy and Clinical Immunology, Vol. 88, No. 2, 1991, p. 166-171.

Research output: Contribution to journalArticle

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