Use of organoids to study regenerative responses to intestinal damage

Sarah E. Blutt, Ophir D. Klein, Mark Donowitz, Noah Shroyer, Chandan Guha, Mary K. Estes

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Intestinal organoid cultures provide an in vitro model system for studying pathways and mechanisms involved in epithelial damage and repair. Derived from either embryonic or induced pluripotent stem cells or adult intestinal stem cells or tissues, these self-organizing, multicellular structures contain polarized mature cells that recapitulate both the physiology and heterogeneity of the intestinal epithelium. These cultures provide a cutting-edge technology for defining regenerative pathways that are induced following radiation or chemical damage, which directly target the cycling intestinal stem cell, or damage resulting from viral, bacterial, or parasitic infection of the epithelium. Novel signaling pathways or biological mechanisms identified from organoid studies that mediate regeneration of the epithelium following damage are likely to be important targets of preventive or therapeutic modalities to mitigate intestinal injury. The evolution of these cultures to include more components of the intestinal wall and the ability to genetically modify them are key components for defining the mechanisms that modulate epithelial regeneration.

Original languageEnglish (US)
Pages (from-to)G845-G852
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume317
Issue number6
DOIs
StatePublished - Dec 2019
Externally publishedYes

Keywords

  • Enteroids
  • Intestinal stem cell
  • Organoids
  • Regeneration

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Use of organoids to study regenerative responses to intestinal damage'. Together they form a unique fingerprint.

Cite this