Use of GLUT-4 null mice to study skeletal muscle glucose uptake

Maureen J. Charron, Naira Gorovits, J. Skye Laidlaw, Mollie Ranalletta, Ellen B. Katz

Research output: Contribution to journalReview article

15 Scopus citations

Abstract

1. The present review focuses on the effects of varying levels of GLUT-4, the insulin-senstive glucose transporter, on insulin sensitivity and whole body glucose homeostasis. 2. Three mouse models are discussed including myosin light chain (MLC)-GLUT-4 mice which overexpress GLUT-4 specifically in skeletal muscle, GLUT-4 null mice which express no GLUT-4 and the MLC-GLUT-4 null mice which express GLUT-4 only in skeletal muscle. Overexpressing GLUT-4 specifically in the skleletal muscle results in increased insulin sensitivity in the MLC-GLUT-4 mice. In contrast, the GLUT-4 null mice exhibit insulin intolerance accompanied by abnormalities in glucose and lipid metabolism. Restoring GLUT-4 expression in skeletal muscle in the MLC-GLUT-4 null mice results in normal glucose metabolism but continued abnormal lipid metabolism. 3. The results of experiments using these mouse models demonstrates that modifying the expresssion of GLUT-4 profoundly affects whole body insulin action and consequently glucose and lipid metabolism.

Original languageEnglish (US)
Pages (from-to)308-313
Number of pages6
JournalClinical and Experimental Pharmacology and Physiology
Volume32
Issue number4
DOIs
StatePublished - Apr 2005

Keywords

  • GLUT-4
  • Glucose transporter
  • Glucose uptake
  • Insulin action
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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