Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAFV600E mutation

Susana García-Silva; Alberto Benito-Martín; Sara Sánchez-Redondo; Alberto Hernández-Barranco; Pilar Ximénez-Embún; Laura Nogués; Marina S. Mazariegos; Kay Brinkmann; Ana Amor López; Lisa Meyer; Carlos Rodríguez; Carmen García-Martín; Jasminka Boskovic; Rocío Letón; Cristina Montero; Mercedes Robledo; Laura Santambrogio; Mary Sue Brady; Anna Szumera-Ciećkiewicz; Iwona Kalinowska; Johan Skog; Mikkel Noerholm; Javier Muñoz; Pablo L. Ortiz-Romero; Yolanda Ruano; José L. Rodríguez-Peralto; Piotr Rutkowski; Héctor Peinado

doi:10.1084/jem.20181522

Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF^V600E mutation

Susana García-Silva, Alberto Benito-Martín, Sara Sánchez-Redondo, Alberto Hernández-Barranco, Pilar Ximénez-Embún, Laura Nogués, Marina S. Mazariegos, Kay Brinkmann, Ana Amor López, Lisa Meyer, Carlos Rodríguez, Carmen García-Martín, Jasminka Boskovic, Rocío Letón, Cristina Montero, Mercedes Robledo, Laura Santambrogio, Mary Sue Brady, Anna Szumera-Ciećkiewicz, Iwona KalinowskaJohan Skog, Mikkel Noerholm, Javier Muñoz, Pablo L. Ortiz-Romero, Yolanda Ruano, José L. Rodríguez-Peralto, Piotr Rutkowski, Héctor Peinado

Pathology

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAF^V600E mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse.

Original language	English (US)
Pages (from-to)	1061-1070
Number of pages	10
Journal	Journal of Experimental Medicine
Volume	216
Issue number	5
DOIs	https://doi.org/10.1084/jem.20181522
State	Published - May 1 2019

ASJC Scopus subject areas

Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1084/jem.20181522

Cite this

García-Silva, S., Benito-Martín, A., Sánchez-Redondo, S., Hernández-Barranco, A., Ximénez-Embún, P., Nogués, L., Mazariegos, M. S., Brinkmann, K., López, A. A., Meyer, L., Rodríguez, C., García-Martín, C., Boskovic, J., Letón, R., Montero, C., Robledo, M., Santambrogio, L., Brady, M. S., Szumera-Ciećkiewicz, A., ... Peinado, H. (2019). Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF^V600E mutation. Journal of Experimental Medicine, 216(5), 1061-1070. https://doi.org/10.1084/jem.20181522

García-Silva, S, Benito-Martín, A, Sánchez-Redondo, S, Hernández-Barranco, A, Ximénez-Embún, P, Nogués, L, Mazariegos, MS, Brinkmann, K, López, AA, Meyer, L, Rodríguez, C, García-Martín, C, Boskovic, J, Letón, R, Montero, C, Robledo, M, Santambrogio, L, Brady, MS, Szumera-Ciećkiewicz, A, Kalinowska, I, Skog, J, Noerholm, M, Muñoz, J, Ortiz-Romero, PL, Ruano, Y, Rodríguez-Peralto, JL, Rutkowski, P & Peinado, H 2019, 'Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF^V600E mutation', Journal of Experimental Medicine, vol. 216, no. 5, pp. 1061-1070. https://doi.org/10.1084/jem.20181522

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abstract = "Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAFV600E mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse.",

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AU - Hernández-Barranco, Alberto

AU - Ximénez-Embún, Pilar

AU - Nogués, Laura

AU - Mazariegos, Marina S.

AU - Brinkmann, Kay

AU - López, Ana Amor

AU - Meyer, Lisa

AU - Rodríguez, Carlos

AU - García-Martín, Carmen

AU - Boskovic, Jasminka

AU - Letón, Rocío

AU - Montero, Cristina

AU - Robledo, Mercedes

AU - Santambrogio, Laura

AU - Brady, Mary Sue

AU - Szumera-Ciećkiewicz, Anna

AU - Kalinowska, Iwona

AU - Skog, Johan

AU - Noerholm, Mikkel

AU - Muñoz, Javier

AU - Ortiz-Romero, Pablo L.

AU - Ruano, Yolanda

AU - Rodríguez-Peralto, José L.

AU - Rutkowski, Piotr

AU - Peinado, Héctor

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