Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation

Susana García-Silva, Alberto Benito-Martín, Sara Sánchez-Redondo, Alberto Hernández-Barranco, Pilar Ximénez-Embún, Laura Nogués, Marina S. Mazariegos, Kay Brinkmann, Ana Amor López, Lisa Meyer, Carlos Rodríguez, Carmen García-Martín, Jasminka Boskovic, Rocío Letón, Cristina Montero, Mercedes Robledo, Laura Santambrogio, Mary Sue Brady, Anna Szumera-Ciećkiewicz, Iwona KalinowskaJohan Skog, Mikkel Noerholm, Javier Muñoz, Pablo L. Ortiz-Romero, Yolanda Ruano, José L. Rodríguez-Peralto, Piotr Rutkowski, Héctor Peinado

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAF V600E mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse.

Original languageEnglish (US)
Pages (from-to)1061-1070
Number of pages10
JournalJournal of Experimental Medicine
Volume216
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Seroma
Drainage
Melanoma
Biomarkers
Mutation
Exosomes
Lymph Node Excision
Proteomics
Neoplasms
Biopsy
Recurrence
Extracellular Vesicles
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

García-Silva, S., Benito-Martín, A., Sánchez-Redondo, S., Hernández-Barranco, A., Ximénez-Embún, P., Nogués, L., ... Peinado, H. (2019). Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation Journal of Experimental Medicine, 216(5), 1061-1070. https://doi.org/10.1084/jem.20181522

Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation . / García-Silva, Susana; Benito-Martín, Alberto; Sánchez-Redondo, Sara; Hernández-Barranco, Alberto; Ximénez-Embún, Pilar; Nogués, Laura; Mazariegos, Marina S.; Brinkmann, Kay; López, Ana Amor; Meyer, Lisa; Rodríguez, Carlos; García-Martín, Carmen; Boskovic, Jasminka; Letón, Rocío; Montero, Cristina; Robledo, Mercedes; Santambrogio, Laura; Brady, Mary Sue; Szumera-Ciećkiewicz, Anna; Kalinowska, Iwona; Skog, Johan; Noerholm, Mikkel; Muñoz, Javier; Ortiz-Romero, Pablo L.; Ruano, Yolanda; Rodríguez-Peralto, José L.; Rutkowski, Piotr; Peinado, Héctor.

In: Journal of Experimental Medicine, Vol. 216, No. 5, 01.05.2019, p. 1061-1070.

Research output: Contribution to journalArticle

García-Silva, S, Benito-Martín, A, Sánchez-Redondo, S, Hernández-Barranco, A, Ximénez-Embún, P, Nogués, L, Mazariegos, MS, Brinkmann, K, López, AA, Meyer, L, Rodríguez, C, García-Martín, C, Boskovic, J, Letón, R, Montero, C, Robledo, M, Santambrogio, L, Brady, MS, Szumera-Ciećkiewicz, A, Kalinowska, I, Skog, J, Noerholm, M, Muñoz, J, Ortiz-Romero, PL, Ruano, Y, Rodríguez-Peralto, JL, Rutkowski, P & Peinado, H 2019, ' Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation ', Journal of Experimental Medicine, vol. 216, no. 5, pp. 1061-1070. https://doi.org/10.1084/jem.20181522
García-Silva S, Benito-Martín A, Sánchez-Redondo S, Hernández-Barranco A, Ximénez-Embún P, Nogués L et al. Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation Journal of Experimental Medicine. 2019 May 1;216(5):1061-1070. https://doi.org/10.1084/jem.20181522
García-Silva, Susana ; Benito-Martín, Alberto ; Sánchez-Redondo, Sara ; Hernández-Barranco, Alberto ; Ximénez-Embún, Pilar ; Nogués, Laura ; Mazariegos, Marina S. ; Brinkmann, Kay ; López, Ana Amor ; Meyer, Lisa ; Rodríguez, Carlos ; García-Martín, Carmen ; Boskovic, Jasminka ; Letón, Rocío ; Montero, Cristina ; Robledo, Mercedes ; Santambrogio, Laura ; Brady, Mary Sue ; Szumera-Ciećkiewicz, Anna ; Kalinowska, Iwona ; Skog, Johan ; Noerholm, Mikkel ; Muñoz, Javier ; Ortiz-Romero, Pablo L. ; Ruano, Yolanda ; Rodríguez-Peralto, José L. ; Rutkowski, Piotr ; Peinado, Héctor. / Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation In: Journal of Experimental Medicine. 2019 ; Vol. 216, No. 5. pp. 1061-1070.
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