Use of cDNA arrays to explore gene expression in genetically manipulated mice and cell lines

Dumitru A. Iacobas, Sandra Iacobas, David C. Spray

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Mutations and altered expression of specific genes have been shown to be responsible for a number of cardiovascular disorders, including chronic hypertension, coronary heart disease, stroke, and rheumatic heart disease as well as arrhythmias, congenital defects and diseases of the vessel wall. For many of these disorders, changes in gene expression leads to deleterious alterations of protein concentrations that may be compensated by therapeutic treatment. Identification of such changes in disease states may thus help indicate treatment strategies to prevent the disease or diminish its severity. An important tool for determining the impact of altered expression of selected genes is the genetically manipulated animal model, in which one or more genes are knocked out or knocked in. A relatively recent interest of our group has been to evaluate alterations in gene expression in various tissues of mice, in which the coding region of particular gap junction proteins (connexins) is disrupted by homologous recombination and in cultures of connexin-deficient cell lines transiently or stably transfected with connexins.

Original languageEnglish (US)
Title of host publicationPractical Methods in Cardiovascular Research
PublisherSpringer Berlin Heidelberg
Pages907-915
Number of pages9
ISBN (Print)3540407634, 9783540407638
DOIs
StatePublished - 2005

Fingerprint

Connexins
Oligonucleotide Array Sequence Analysis
Gene Expression
Cell Line
Rheumatic Heart Disease
Public Opinion
Homologous Recombination
Coronary Disease
Cardiac Arrhythmias
Animal Models
Stroke
Hypertension
Mutation
Genes
Proteins
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Iacobas, D. A., Iacobas, S., & Spray, D. C. (2005). Use of cDNA arrays to explore gene expression in genetically manipulated mice and cell lines. In Practical Methods in Cardiovascular Research (pp. 907-915). Springer Berlin Heidelberg. https://doi.org/10.1007/3-540-26574-0_45

Use of cDNA arrays to explore gene expression in genetically manipulated mice and cell lines. / Iacobas, Dumitru A.; Iacobas, Sandra; Spray, David C.

Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg, 2005. p. 907-915.

Research output: Chapter in Book/Report/Conference proceedingChapter

Iacobas, DA, Iacobas, S & Spray, DC 2005, Use of cDNA arrays to explore gene expression in genetically manipulated mice and cell lines. in Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg, pp. 907-915. https://doi.org/10.1007/3-540-26574-0_45
Iacobas DA, Iacobas S, Spray DC. Use of cDNA arrays to explore gene expression in genetically manipulated mice and cell lines. In Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg. 2005. p. 907-915 https://doi.org/10.1007/3-540-26574-0_45
Iacobas, Dumitru A. ; Iacobas, Sandra ; Spray, David C. / Use of cDNA arrays to explore gene expression in genetically manipulated mice and cell lines. Practical Methods in Cardiovascular Research. Springer Berlin Heidelberg, 2005. pp. 907-915
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